Altered expression of miR-125a and dysregulated cytokines in systemic lupus erythematosus: Unveiling diagnostic and prognostic markers

Table 1 The association of microRNA-125a, tumor necrosis factor-alpha, and interleukin 12 with major clinical symptoms of systemic lupus erythematosus patients

Characteristics		miR-125a		TNF-α		IL-12
Lupus nephritis	Yes	0.199 ± 0.07	P = 0.576	53.42 ± 14.44	P = 0.153	122.37 ± 22.36	P = 0.104
	No	0.205 ± 0.09		45.60 ± 12.13		108.34 ± 29.87
Malar rash	Yes	0.180 ± 0.06	P = 0.017	54.60 ± 12.44	P = 0.360	128.45 ± 20.28	P = 0.065
	No	0.216 ± 0.09		43.37 ± 11.51		102.42 ± 28.94
Hair loss	Yes	0.188 ± 0.06	P = 0.022	52.63 ± 13.08	P = 0.401	126.87 ± 20.62	P = 0.049
	No	0.211 ± 0.09		44.34 ± 11.98		103.19 ± 29.48
SLEDAI	≤ 4	0.202 ± 0.08	P = 0.005	44.00 ± 14.71	P = 0.001	102.43 ± 23.38	P = 0.000
	5-12	0.280 ± 0.08		30.49 ± 12.59		69.54 ± 32.43
	≥ 12	0.199 ± 0.09		49.11 ± 13.51		113.26 ± 28.54

Table 2 List of primers for microRNA-125a and U6 internal control

Primer	Sequence (5'>3')
miR-125a	F: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGT
	R: GTCGTATCCAGTGCAGGGTCCGAGGTGCCGAGGATTTCCACCACCTG
U6	F: GCTTCGGCAGCACATATACTAAAAT
	R: CGCTTCACGAATTTGCGTGTCAT