Concordance of programmed death-ligand 1 expression assessments determined via two immunohistochemical tests and the polymerase chain reaction method

Figure 1 Distribution of patients with breast cancer. A: According to T and N stages and tumor grade; B: According to their molecular and biological characteristics. Abbreviations: ER: Estrogen receptor; HER2: Human epidermal growth factor receptor-2; PR: Progesterone receptor; TNBC: Triple negative breast cancer.

Figure 2 Assessment of tumor-infiltrating programmed cell death protein 1 ligand 1-positive and SP142+ immune cells. A: Programmed cell death protein 1 ligand 1-positive (PDCD1 LG1+) immune cell (IC) score < 10%; B: PDCD1 LG1+ IC score ≥ 10%; C: SP142 IC score < 1%; D: SP142+ IC score ≥ 1%. Staining with anti-PDCD1 LG1 antibodies (A and B), 200 ×; staining with anti-programmed death-ligand 1 antibodies (clone SP142)(C and D), 200 ×.

Figure 3 Programmed death-ligand 1 expression in benign tonsil tissue. A: Staining with anti-programmed cell death protein 1 Ligand 1 antibody, 200 ×; B: Staining with anti-programmed death-ligand 1 (clone SP142) antibody, 200 ×.

Figure 4 Receiver operating characteristic curve discriminating programmed cell death protein 1 Ligand 1 scores corresponding to positive and negative programmed death-ligand 1 (clone SP142) status (< 1% and ≥ 1%). AUC: Area under the curve.

Figure 5 Frequencies of programmed death-ligand 1 expression in immune cells according to marker determination methods. A: Frequencies of programmed death-ligand 1 (PD-L1) expression for all three markers and their combinations; B: Frequencies of programmed cell death protein 1 Ligand 1 (PDCD1 LG1) and PD-L1 (clone SP142) expression and their combination; C: Frequencies of PDCD1 LG1 and PD-L1 [polymerase chain reaction (PCR)] expression and their combination; D: Frequencies of PD-L1 (clone SP142) and PD-L1 (PCR) expression and their combination. PCR: Polymerase chain reaction; PDCD1 LG1: Programmed cell death protein 1 Ligand 1; PD-L1: Programmed death-ligand 1.