Impact of cell death manipulation on the efficacy of photodynamic therapy-generated cancer vaccines

Table 1 Changes in the therapeutic efficacy of photodynamic therapy-generated vaccines induced by modulators of cell death

Cell death modulating agent	Impact on therapeutic efficacy of PDT-generated cancer vaccine
Z-DEVD-FMK (apoptosis inhibitor)	Reduces
IM-54 (necrosis inhibitor)	Enhances
Necrostatin-1 (necroptosis inhibitor)	Enhances
Necrostatin-7 (necroptosis inhibitor)	Enhances
STF62247 (lethal autophagy inducer)	Enhances
Spautin-1 (lethal autophagy inhibitor)	Reduces

Mice with growing SCCVII tumors (murine squamous cell carcinoma) were vaccinated by SCCVII cells (20 million/mouse) that were first treated by PDT (mediated by photosensitizer ce6) and then incubated before use for 16 h with or without cell death modulating agents[3]. The therapy response was monitored by tumor size measurement in time following vaccine treatment. PDT: Photodynamic therapy.