Ozone action on Streptococcus mutans and Lactobacillus fermentum: A pilot study

Table 1 Composition of artificial saliva, modification of basal medium mucin Medium with modified artificial Shellis saliva

	g/L
Yeast extract	5
Proteose peptone	10
Potassium chloride (KCl; 74.5 g/mol)	1.2
Sodium hydrogen carbonate (NaHCO3; 84 g/L)	0.6
Potassium thiocyanate (KSCN; 97.18 g/mol)	0.23
Sodium Di-hydrogen phosphate (Na2HPO 4.12 H2O; 358 g/mol)	0.9

Table 2 Results of DIAGNOdent, expressed as mean ± SE, quartiles and range of variation

Material	Med	n	Mean	Structural equation modeling	Min	Max	P25	P50	P75	Med1 vs Med2
Artificial Saliva	1	10	6.10	0.795	2	11	4.75	6.00	7.50	0.759
	2	10	8.20	4.54	0	45	0.00	1.00	14.75
L. fermentum	1	10	5.40	0.792	2	10	3.00	5.50	7.25	0.011
	2	10	19.40	6.31	2	63	7.00	9.50	27.50
S. mutans	1	10	5.60	0.859	2	9	2.00	6.50	8.00	0.011
	2	10	30.00	10.33	0	99	9.25	15.50	47.00
S. mutans + L. fermentum	1	10	6.00	0.978	2	12	4.50	5.00	8.50	0.005
	2	10	31.90	3.29	16	47	25.00	29.50	43.75
S. mutans + L. fermentum ozone + remin	1	20	5.15	0.782	1	12	2.25	4.00	8.75	< 0.001
	2	20	45.95	5.90	20	99	24.50	37.00	68.50
Comparisons between 5 groups	1	P = 0.730
	2	P < 0.001

S. mutans: Streptococcus mutans; L. fermentum: Lactobacillus fermentum.

Table 3 Measurements difference for each of 60 teeth

			Group					Total
			Artificial saliva	L. fermentum	S. mutans	S. mutans + L. fermentum	S. mutans + L. fermentum + ozone
Difference	Decreased	n(%)	7	1	1	0	0	9
		Residue	5.5	-0.5	-0.5	-1.5	-3.0
	Maintenance	n(%)	0	1	0	0	0	1
		Residue	-0.2	0.8	-0.2	-0.2	-0.3
	Increased	n(%)	3	8	9	10	20	50
		Residue	-5.3	-0.3	0.7	1.7	3.3
Total			10	10	10	10	20	60

S. mutans:Streptococcus mutans;L. fermentum:Lactobacillus fermentum.