CD34+ dermal dendritic cells and mucin deposition in dermatomyositis

Table 1 Antibodies used in immunohistochemical analysis

Antibody	Type	Source	Dilution
Vimentin	Mouse	Dako	1:40
CD34	Mouse	Nichirei	1:1
Factor XIIIa	Rabbit	Biogenesis	1:50
α-smooth muscle actin	Mouse	Dako	1:50
Desmin	Mouse	Dako	1:1
Tryptase	Mouse	Dako	1:50

Table 2 Profiles of eight patients with dermatomyositi

No	Age	Sex/period	Clinical findings	Sight of skin biopsy	Pathological findings
1	86	Male	Rash erythema of whole body respiratory failure	Right tight	Vacuolar change, mucin in papillary and reticular dermis
		One month
2	59	Female	Lilac rash of eyelids, erythema rash of limbs, dorsal hands of Gottron’s papules arthralgia	Right tight	Vacuolar change, subepidermal blister, mucine in papillary dermis
		One month
3	53	Female	Dorsal hands of Gottron’s papules, scary erythema of face, chest, limbs, arthralgia, muscle weakness	Light tight	Vacuolar change, periadnexal infiltration
		Two months			mucin in papillary and reticular dermis
4	63	Female	Lilac rash of eyelids, dorsal hands of Gottron’s papules, scary erythema of face, neck, chest	Chest	Vacuolar change, periadnexal infiltration mucin in papillary and reticular dermis
		Five months
5	46	Male	Dale reddish erythema of face, edematous erythema of neck	Limbs	Vacuolar change, melanine incontinen mucin in papillary and reticular dermis
		Unidentified
6	34	Female	Dorsal hands of Gottron’s papules rash erythema of eyelid, nasal grooves muscle weakness	Chest	Hyperkeratosis, vacuolar change, mucin in papillary dermis
		Five months
7	46	Female	Dorsal hands of Gottron’s papules, scary erythema of knee and hip, arthralgia	Light dorsal hand	Hyperkeratosis, vacuolar change, mucin in papillary and reticular dermis
		Three months
8	57	Male1	Edematous erythema of face, neck, chest and shoulder	Chest	Vacuolar change, mucin in papillary and reticular dermis
		Six months

¹Only the 57-year-old male (case 8) was found lung cancer, the other seven patients had no internal malignancy.

Table 3 Number of positive cells in normal skin and dermatomyositis

	Normal skin (n = 15) mean ± SE cells/high-power field (× 400)	Dermatomyositis (n = 8) mean ± SE cells/high-power field (× 400)	P value
Mast cells tryptase	12.320 ± 0.997	5.788 ± 0.805	0.000270
Dendritic cells
Vimentin	9.654 ± 1.374	16.0125 ± 3.257	0.0524
CD34	10.933 ± 1.131	14.937 ± 3.257	0.177
Factor XIIIa	8.708 ± 2.172	4.40 ± 1.619	0.166
α-SMA	0	0.488 ± 0.447	0.141
Desmin	0	0