Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs

Figure 1 Condyles of canine thigh bone after experimental modelling of osteoarthrosis (A), scheme of articular cartilage harvesting (B).

Figure 2 X-ray electron probe microanalysis of articular cartilage in intact dog. A: SEM micrograph of articular cartilage and subchondral zone; B: Scanning line regimen, graph shows calcium distribution on line; C: Scanning area regimen shows calcium distribution on the sample surface. Instrumental magnification × 110.

Figure 3 Articular cartilage of intact dog. A: Semithin section, methylene blue stain. Ob. -6.3 х; oc. -12.5 х; B: Smart map shows sulphur distribution on scanned line. Instrumental magnification 170.

Figure 4 Articular cartilage in dog with experimentally induced osteoarthrosis. Methylene blue - basic fuchsin staining. A: General appearance. Ob. - 6.3 x, oc. - 12.5 x; B: Focal basophilia of intercellular matrix in deep zone. Ob. - 100 OI, oc. - 12.5 x; C: Osteoclast, resorbing the ground substance of calcified cartilage. Howship lacuna (arrow). Ob. - 100 OI, oc. - 12.5 x.

Figure 5 X-ray electron probe microanalysis of articular cartilage in dog with experimentally induced osteoarthrosis. A: SEM micrograph of articular cartilage and subchondral zone; B: Scanning line regimen, graph shows calcium distribution on line; C: Scanning area regimen shows calcium distribution on the sample surface. Instrumental magnification 100.

Figure 6 Age-related changes of articular cartilage in intact group. Semithin section. Methylene blue - basic fuchsin staining. A: Focal metachromasy of articular cartilage in five year old dog. Ob. -6,3 х; oc. - 12,5 х; B: Intensive metachromasy in intermediate and deep zones of articular cartilage in eight year old dog. Ob. -16 х; oc. - 12,5 х.

Figure 7 X-ray electron probe microanalysis of articular cartilage in intact five years old dog. A: SEM micrograph of articular cartilage and subchondral zone; B: Scanning line regimen, graph shows phosphorus distribution on line; C: Scanning area regimen shows phosphorus distribution on the sample surface. Instrumental magnification 120.