Basic Study
Copyright ©The Author(s) 2022.
World J Orthop. Jun 18, 2022; 13(6): 587-602
Published online Jun 18, 2022. doi: 10.5312/wjo.v13.i6.587
Figure 1
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Figure 1 Different platelet-rich plasma kits after centrifugation. Highlight specific items in each kit used for platelet-rich plasma (PRP) extraction. A: Arthrex autologous conditioned plasma, the prepared plasma is transferred by pulling it into the inner syringe in a double-syringe system; B: Mini GPS III, PRP is automatically separated during centrifugation by a special membrane in the separator; C: Xerthra, the controller screw placed at the bottom is used to move the PRP to the neck of the tube; D: Dr. PRP, the piston is used to push PRP to the tube’s neck.
Figure 2
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Figure 2 Platelet capture efficiency (%) of different preparation protocols. aP < 0.001 vs Mini GPS III; bP = 0.001 vs Mini GPS III. Arthrex ACP: Arthrex Autologous Conditioned Plasma.
Figure 3
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Figure 3 Concentrations (pg/mL) of growth factors in platelet-rich plasma samples obtained by different systems (mean ± SD). EGF: Epidermal growth factor; FGF-basic: Fibroblast growth factor-basic; HGF: Hepatocyte growth factor; VEGF: Vascular endothelial growth factor; TGF-β1: Transforming growth factor-β1; PDGF-AA: Platelet-derived growth factor-AA; PDGF-BB: Platelet-derived growth factor-BB.
Figure 4
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Figure 4 Concentrations (pg/mL) of inflammatory cytokines in platelet-rich plasma samples obtained by different systems (mean ± SD). A: Mini GPS III; B: Arthrex Autologous Conditioned Plasma. IFN-α2: Interferon-α2; IL-10: Interleukin-10; IL-12p70: Interleukin-12p70; IL-17A: Interleukin-17A; IL18: Interleukin-18; IL-1β: Interleukin-1β; IL-23: Interleukin-23; IL-33: Interleukin-33; Il-6: Interleukin-6; IL-8: Interleukin-8; IFN-γ: Interferon-γ; MCP-1: Monocyte chemoattractant protein-1; TNF-α: Tumor necrosis factor α.
Figure 5
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Figure 5 Spearman correlations (ρ) between platelets and growth factors in platelet-rich plasma obtained with different protocols and combined. aP < 0.05. EGF: Epidermal growth factor; FGF-basic: Fibroblast growth factor-basic; HGF: Hepatocyte growth factor; VEGF: Vascular endothelial growth factor; TGF-β1: Transforming growth factor-β1; PDGF-AA: Platelet-derived growth factor-AA; PDGF-BB: Platelet-derived growth factor-BB.