Cigarette smoking, dietary habits and genetic polymorphisms in GSTT1, GSTM1 and CYP1A1 metabolic genes: A case-control study in oncohematological diseases

Figure 1 Gel electrophoresis showing PCR products of GSTT1 (459 bp), GSTM1 (273 bp) and CLOCK (221 bp). Numbered lanes represents: 1: 100 bp molecular weight marker; 3, 4, 8: T⁺/M⁺; DNA from samples with positive GSTT1 and GSTM1 alleles, plus control CLOCK gene band; 7: T^-/M^-; Double null genotype of GSTM1 and GSTT1 (in the presence of CLOCK PCR product); 5, 6, 9: T⁺/M^-; GSTT1 positive/GSTM1 null; 2: T^-/M⁺; GSTT1 null/GSTM1 positive.

Figure 2 Gel electrophoresis showing PCR-RFLP product of CYP1A1 gene digested with MspI restriction enzyme. CYP1A1 gene T3801C variant gives the following digestion band patterns: TT, 420 bp; TC, 420 bp and 237 bp + 183 bp; and CC, 237 bp + 183 bp. Numbered lanes represents: 1: 100 bp molecular weight marker; 2, 5: CC homozygous type; 3, 4, 7: TC heterozygous type; 6, 8, 9: TT homozygous type.