Checkpoint kinase 1 in colorectal cancer: Upregulation of expression and promotion of cell proliferation

Figure 1 Flowchart of this study. Shows the data analysis and main article structure.

Figure 2 Single-cell analysis of checkpoint kinase 1 expression in Colorectal cancer tissues. A: Cell annotation; B: Expression of checkpoint kinase 1 in epithelial cells; C: Expression of checkpoint kinase 1 in malignant and normal epithelial cells.

Figure 3 Expression of checkpoint kinase 1 protein in adjacent non-cancerous tissues (left side of images: 500 μm, middle column: 200 μm, right side: 50 μm). A: Sample 1; B: Sample 2; C: Sample 3; D: Sample 4.

Figure 4 Expression of checkpoint kinase 1 protein in colorectal cancer tissues (left side of images: 500 μm, middle column: 200 μm, right side: 50 μm). A: Sample 1; B: Sample 2; C: Sample 3; D: Sample 4.

Figure 5 Internal tissue microarray analysis of checkpoint kinase 1 expression in colorectal cancer. A: Violin plot; B: Receiver operating characteristic curve. TMA: Tissue microarray; TPR: True positive rate; FPR: False positive rate; AUC: Area under the curve.

Figure 6 Flowchart of the selection process for the checkpoint kinase 1 mRNA dataset. CHEK1: Checkpoint kinase 1.

Figure 7 Differential expression of checkpoint kinase 1 in colorectal cancer samples vs non-colorectal cancer samples within the included datasets. CRC: Colorectal cancer; AUC: Area under the curve.

Figure 8 Continued analysis of the differential expression of checkpoint kinase 1 in colorectal cancer samples compared to non-colorectal cancer samples within the included datasets. CRC: Colorectal cancer; AUC: Area under the curve.

Figure 9 Comprehensive analysis of checkpoint kinase 1 expression in colorectal cancer. A: Random effects model analysis; B: Begg's test for publication bias; C: Egger's test for publication bias.

Figure 10  Quantitative evaluation of checkpoint kinase 1 characteristics in colorectal cancer. A: Summary Receiver Operating Characteristic curve; B: Sensitivity; C: Specificity; D: Positive diagnostic likelihood ratio; E: Negative diagnostic likelihood ratio.

Figure 11  Molecular docking model of nitidine chloride with checkpoint kinase 1 (binding affinity = −11. 8 kcal/mol).

Figure 12  Impact of checkpoint kinase 1 on the proliferation of colorectal cancer cell lines. Each row represents a colorectal cancer cell line, with corresponding gene effect scores at the end.