Prognostic and immunological roles of heat shock protein A4 in lung adenocarcinoma

Figure 1 The results of differentially expressed genes analysis. A: The volcano plot of differentially expressed RNAs; B and C: The different expressions of HSPA4 between lung adenocarcinoma and adjacent normal tissues; D: Heatmap of the 25 genes correlated to HSPA4. Statistical significance is expressed as ^cP < 0.0001.

Figure 2 Enrichment analysis of HSPA4 in lung adenocarcinoma. A: Biological process enrichment related to HSPA4-related genes; B: A network of HSPA4 and its 10 potential co-interaction proteins; C and D: The results of enrichment analysis from gene set enrichment analysis.

Figure 3 The results of analysis between HSPA4 expression and immune infiltration. A: The negative correlation between HSPA4 expression and T cells; B: CD8 T cells; C: B cells; D: Cytotoxic cells; E: The negative correlation between HSPA4 expression and Th2 cells; F: T helper cells; G: Correlation between HSPA4 expression level and the relative abundances of 24 immune cells; H: Heatmap of 24 immune infiltration cells in lung adenocarcinoma.

Figure 4 Correlation between the HSPA4 expression and different clinicopathologic characteristics. A: Correlation between the HSPA4 expression and the pathologic T stage; B: N stage; C: M stage; D: Pathologic stage; E: Primary therapy outcome; F: Gender. Statistical significance is expressed as ^aP < 0.05, ^bP < 0.01, ^cP < 0.0001.

Figure 5 The prognostic value of HSPA4 in lung adenocarcinoma. A: Multivariate Cox regression visualized in the forest plot; B: HSPA4 expression distribution and survival status. 0: dead, 1: alive; C: Nomogram with the HSPA4 expression and traditional clinical prognostic factors.

Figure 6 The prognostic value of HSPA4 in the different subgroups. A and B: The prognostic value of HSPA4 in overall survival and disease specific survival of lung adenocarcinoma patients; C–H: High expression of HSPA4 was associated with worse overall survival in different subgroups.

Figure 7 The prognostic value of HSPA4 in three independent datasets. A: The prognostic value of HSPA4 in GSE31210 database; B: GSE13213 database; C: HARVARD-LC database.

Figure 8 Visualization between the methylation level and HSPA4 expression.