Novel molecular panel for evaluating systemic inflammation and survival in therapy naïve glioma patients

Figure 1 Qualitative screening of kynurenine by dot–immune binding assay in control, grade II, grade III, and grade IV samples; red arrow indicates the presence of immune-complex. A: Control; B: Grade II; C: Grade III; D: Grade IV. KYN: Kynurenine; CON: Control; G: Grade.

Figure 2 Survival curves of circulatory markers kynurenine, interleukin-6, neutrophil-lymphocyte ratio and human telomerase reverse transcriptase(red indicates worse prognosis while green represents favorable prognosis), differential survival between astrocytic isocitrate dehydrogenase-mutant and isocitrate dehydrogenase-wildtype gliomas, combination receiver operating characteristic of the panel of kynurenine, interleukin-6, neutrophil-lymphocyte ratio, and human telomerase reverse transcriptase to achieve increased sensitivity and specificity for predicting overall survival. A: Kynurenine; B: Interleukin-6; C: Neutrophil-lymphocyte ratio; D: Human telomerase reverse transcriptase; E: Differential survival between astrocytic isocitrate dehydrogenase-mutant and wildtype gliomas; F: Combination receiver operating characteristic of the panel of kynurenine, interleukin-6, neutrophil-lymphocyte ratio, and human telomerase reverse transcriptase to achieve increased sensitivity and specificity for predicting overall survival. KYN: Kynurenine; IL-6: Interleukin-6; hTERT: Human telomerase reverse transcriptase; NLR: Neutrophil- lymphocyte ratio; IDH-m astro: Isocitrate dehydrogenase mutant astrocytic; IDH-w astro: Isocitrate dehydrogenase wildtype astrocytic.

Figure 3 A non-invasive model for evaluating chronic systemic inflammation and overall survival in glioma. KYN: Kynurenine; IL-6: Interleukin-6; NLR: Neutrophil lymphocytes ratio; hTERT: Human telomerase reverse transcriptase; ELISA: Enzyme-linked immunosorbent assay.