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World J Clin Oncol. Jun 10, 2012; 3(6): 82-91
Published online Jun 10, 2012. doi: 10.5306/wjco.v3.i6.82
Published online Jun 10, 2012. doi: 10.5306/wjco.v3.i6.82
Hypoxia and cytokines regulate carbonic anhydrase 9 expression in hepatocellular carcinoma cells in vitro
Feray Kockar, Hatice Yildrim, Department of Biology, Faculty of Art and Science, Balikesir University, 10145 Balikesir, Turkey
Rahsan Ilikci Sagkan, Department of Immunology and Allergy Diseases, Gulhane Military Medical Academy, Etlik, 06018 Ankara, Turkey
Carsten Hagemann, Tumorbiology Laboratory, Department of Neurosurgery, University of Würzburg, D-97080 Würzburg, Germany
Yasemin Soysal, Faculty of Medicine, Department of Medical Genetics, Afyon Kocatepe University, 03100 Afyonkarahisar, Turkey
Jelena Anacker, Department of Obstetrics and Gynaecology, University of Würzburg, D-97080 Würzburg, Germany
Ahmed Ayad Hamza, School of Medicine, Southern Illinois University, Springfield, IL 62794-9620, United States
Dirk Vordermark, Department of Radiation Oncology, University-Halle-Wittenberg, 06110 Halle, Germany
Michael Flentje, Harun M Said, Department of Radiation Oncology, University of Wuerzburg, D-97080 Würzburg, Germany
Author contributions: Kockar F and Said HM were the primary authors and performed the in vitro hypoxia experiments, supplied the in vitro mRNA, protein lysates and nuclear extracts, performed the western blots, densitometric analysis of the results and participated in the study design; Hagemenn C, Soysal Y, Hamza AA were co-authors and participated in the study design; Kockar F and Said HM coordinated the group and contributed to the development of the experimental strategy; Anacker J designed the primers used for reverse transcription polymerase chain reaction and participated in the study design and evaluation; Hagemenn C, Vordermark D and Flentje M also participated in the study design; and all authors read and approved the manuscript.
Supported by Deutsche Forschungsgemeinschaft DFG, VO 871/2-3, to Vordermark D; and the IZKF Würzburg, B25, to Hagemenn C; Turkish Research Council (TUBITAK) Project, TBAG 105T326, to Kockar F and Yildrim H; Balikesir University Research Project, 2008/15, to Sagkan RI
Correspondence to: Dr. Harun M Said, PhD, Department of Radiation Oncology, University of Wurzburg, Josef-Schneider-Str. 11, D-97080 Wurzburg, Germany. said@scientist.com
Telephone: +49-16-37531174 Fax: +49-16-37531174
Received: August 30, 2011
Revised: May 30, 2012
Accepted: June 5, 2012
Published online: June 10, 2012
Revised: May 30, 2012
Accepted: June 5, 2012
Published online: June 10, 2012
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