Protective role of Juniperus phoenicea and Cupressus sempervirens against CCl4

Table 1 Effect of Juniperus phoenicea and Cupressus sempervirens leaves on the levels of some biochemical parameters in CCl₄-toxicated serum of rats

Parameters	Control	CCl4-toxicated	CCl4-self recovery	Treated groups		Improvement (%)			ANOVA (P)
				Ext1	Ext2
	G1	G2	G3	G4	G5	G3	G4	G5
Total protein (TP)	7.28 ± 0.61 (2)	5.458 ± 0.55 (1,3,4,5)	6.95 ± 0.28 (2)	6.94 ± 0.43 (2)	7.18 ± 0.42 (2)	20.5	20.83	23.7	< 0.0001
Albumin (Alb)	2.86 ± 0.45 (NS)	2.64 ± 0.11 (NS)	2.69 ± 0.12 (NS)	2.73 ± 0.31 (NS)	2.84 ± 0.24 (NS)	1.74	3.41	7.0	< 0.348
Urea	0.606 ± 0.071 (2)	0.9809 ± 0.15 (1,3,4,5)	0.620 ± 0.053 (2)	0.619 ± 0.1102 (2)	0.613 ± 0.0759 (2)	59.6	59.7	59.7	< 0.0001
Creatinine	0.293 ± 0.079 (2)	0.480 ± 0.08 (1,3,4,5)	0.338 ± 0.07 (2)	0.353 ± 0.021 (2)	0.294 ± 0.065 (2)	48.7	52.73	63.6	< 0.0001
Activity (LDH)	0.098 ± 0.028 (2)	0.495 ± 0.242 (1,3,4,5)	0.1237 ± 0.019 (2)	0.104 ± 0.0412 (2)	0.100 ± 0.017 (2)	479.6	499.59	503.8	< 0.0001

Data are expressed as mean ± SD of ten rats in each group. Values of total protein are expressed as g/dL and Albumin are expressed as g/L; Urea and Creatinine are expressed as mg/dL; Lactate dehydrogenase (LDH) are expressed as U/mL. P is level of significance, where P < 0.0001 is significant. Analysis of data is carried out by one way (ANOVA) (analysis of variance) accompanied by post hoc (LSD) (least significant difference) (SPSS computer programme). Ext₁ means CCl₄ + Me-OH extract of Juniperus phoenicea leaves and Ext₂ means CCl₄ + Me-OH extract of Cupressus sempervirens. NS: Not significant.

Table 2 Effect of Juniperus phoenicea and Cupressus sempervirens leaves on the level of lactate dehydrogenase isoenzymes of CCl₄-toxicated serum of rats

Parameters	Control	CCl4-toxicated	CCl4-self recovery	Treated groups		Improvement (%)			ANOVA (P)
				Ext1	Ext2
	G1	G2	G3	G4	G5	G3	G4	G5
LDH1	0.0036 ± 0.0009 (2)	0.0193 ± 0.017 (1,3,4,5)	0.00767 ± 0.0028 (2)	0.00775 ± 0.0052 (2)	0.00904 ± 0.0056 (2)	324.86	322.63	286.596	< 0.037
LDH2	0.012 ± 0.004 (2)	0.0279 ± 0.0179 (1,5)	0.0165 ± 0.0039 (NS)	0.0161 ± 0.0131 (NS)	0.0086 ± 0.006 (2)	96.61	100	163.56	< 0.044
LDH3	0.0131 ± 0.0075 (NS)	0.0199 ± 0.010 (3,4,5)	0.0100 ± 0.0025 (2)	0.0106 ± 0.0094 (2)	0.00727 ± 0.0014 (2)	75.57	70.99	96.41	< 0.057
LDH4	0.00813 ± 0.00479 (2)	0.0411 ± 0.0314 (1,3,4,5)	0.0106 ± 0.0035 (2)	0.0102 ± 0.0063 (2)	0.0123 ± 0.0054 (2)	375.15	380.07	354.24	< 0.003
LDH5	0.0673 ± 0.0186 (2)	0.409 ± 0.217 (1,3,4,5)	0.0866 ± 0.0189 (2)	0.081 ± 0.008 (2)	0.066 ± 0.019 (2)	479.02	487.19	510.37	< 0.0001

Data are expressed as mean ± SD of ten rats in each group. Values of lactate dehydrogenase isoenzyme (LDH₁) are expressed as U/mL. P is level of significance, where P < 0.0001 is significant. Analysis of data is carried out by one way (ANOVA) (analysis of variance) accompanied by post hoc (LSD) (least significant difference) (SPSS computer programme). Ext₁ means CCl₄ + Me-OH extract of Juniperus phoenicea leaves and Ext₂ means CCl₄ +Me-OH extract of Cupressus sempervirens. NS: Not significant.