Prediction of hereditary nonpolyposis colorectal cancer using mRNA MSH2 quantitative and the correlation with nonmodifiable factor

Table 1 Baseline characteristic and MSH2 gene expression

Characteristic	CRC group	Control group	P value
Age (yr)	56.8 ± 8.4	51.6 ± 13.4	> 0.05
Sex
Male	21 (52.5)	13 (41.9)
Female	19 (47.5)	18 (58.1)
Body mass index (kg/m2)	22.4 ± 3.3	23.6 ± 3.4

CRC: Colorectal cancer.

Table 2 MSH2 gene expression

MSH2 expression	CRC group	Control group	P value
Blood
Median (range)	12554.50 (4230.00-14559.00)	12146.00 (11029.00-13633.00)	0.116
mean ± SD	11411.05 ± 2912.45	12219.87 ± 756.87	0.465
Tissue	7 485.00 (4174.00-14218.00)

CRC: Colorectal cancer; SD: Standard deviation.

Table 3 Colorectal cancer hereditary risk factors

Factor	CRC hereditary, yes (n: 13)	CRC hereditary, no (n: 27)	OR (95%CI)	P value
Age	50.69 ± 14.99	59.74 ± 11.68		0.043
< 50	8 (61.54)	6 (22.22)	5.60 (1.33-23.62)	0.031
> 50	5 (38.46)	21 (77.78)
Gender			0.69 (0.18-2.59)	0.826
Male	6 (46.15)	15 (55.56)
Female	7 (53.85)	12 (44.44)
Location			-	0.595
Caecum	1 (7.69)	1 (3.70)
Ascending colon	2 (15.38)	4 (14.81)
Transverse colon	2 (15.38)	2 (7.41)
Descending colon	0 (0.00)	1 (3.70)
Sigmoid	3 (23.08)	7 (25.93)
Rectum	5 (38.46)	12 (44.44)
Proximity			1.79 (0.44-7.32)	0.476
Proximal colon	5 (38.46)	7 (25.93)
Distal colon	8 (61.54)	20 (74.07)
Staging			-	0.020
A	0 (0.00)	7 (25.93)
B	4 (30.77)	10 (37.04)
C	7 (54.85)	9 (33.33)
D	2 (15.38)	1 (3.70)
Group staging			3.83 (0.93-15.72)	0.116
C-D	9 (69.23)	10 (37.04)
A-B	4 (30.77)	17 (62.96)
Family history			9.20 (1.97-42.97)	0.008
Yes	8 (61.54)	4 (14.81)
No	5 (38.46)	23 (85.19)
Differentiation1
Poor	4 (30.77)	3 (11.54)	2.52 (0.46-13.80)	0.287
Intermediate	0 (0.00)	6 (23.08)	-	0.999
Well	9 (69.23)	17 (65.38)

¹Loss of differentiation data in one sample. CRC: Colorectal cancer; OR: Odds ratio; CI: Confidence interval.

Table 4 Multivariate regression of colorectal cancer hereditary risk factor

Factor	B	SE	P value	OR (95%CI)
Staging	2.395	1.130	0.034	10.970 (1.199-100.382)
History	3.126	1.143	0.006	22.784 (2.423-214.273)
Constant	-3.165	1.094	0.004	0.042

SE: Standard error; OR: Odds ratio; CI: Confidence interval.

Table 5 Scenarios of colorectal cancer hereditary probability

Scenario	Family history1	Staging2	LOGIT3	Probability4
1	1	1	2.356	0.913
2	1	0	-0.039	0.490
3	0	1	-0.770	0.316
4	0	0	-3.165	0.041

¹1: Yes, 0: No.

²1: C or D, 0: A or B.

³LOGIT: -3.165 + 2.395 × staging + 3.126 × history.

⁴Probability: 1/(1 + e^-LOGIT).