Calpain system and its involvement in myocardial ischemia and reperfusion injury

Table 1 Effect of calpain inhibitor A-705239 on impairment of mitochondrial function following myocardial ischemia and reperfusion[180]

	n	State 3 respiration (nmol O2/min per milligram)	State 4 respiration (nmol O2/min per milligram)	RCI (state3 rate): (state 4 rate)	Leak respiration(nmol O2/min per milligram)	Stimulation by cytochrome c %
Control
Before ischemia	4	6.4 ± 1.1	0.5 ± 0.1	12.5 ± 2.7	0.15 ± 0.07	6.0 ± 10.0
Ischemia 45 min	8	3.5 ± 1.4ac	0.9 ± 0.3a	4.4 ± 2.5a	0.32 ± 0.14a	10.0 ± 6.0
Reperfusion 60 min	4	2.6 ± 1.3ac	0.9 ± 0.3a	3.2 ± 2.1a	0.43 ± 0.29	28.0 ± 16.0
A-705239 treated hearts
Before ischemia	4	6.8 ± 1.3	0.6 ± 0.1	12.4 ± 1.1	0.12 ± 0.06	16.0 ± 9.0
Ischemia 45 min	9	5.0 ± 0.8ac	0.6 ± 0.2	8.2 ± 2.3ac	0.20 ± 0.14a	15.0 ± 13.0
Reperfusion 60 min	5	4.2 ± 1.2ac	0.7 ± 0.2	6.4 ± 2.7a	0.26 ± 0.24

Data are presented as means of 4 to 9 experiments mean ± SD measured as duplicates or triplicates. A significant difference from baseline before ischemia is represented by

^aP < 0.05, and between both groups by

^cP < 0.05.