Control of nuclear-cytoplasmic shuttling of Ankrd54 by PKCδ

doi:10.4331/wjbc.v8.i3.163

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World Journal of Biological Chemistry

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1949-8454

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Biol Chem. Aug 26, 2017; 8(3): 163-174
Published online Aug 26, 2017. doi: 10.4331/wjbc.v8.i3.163

Table 1 Multiple reaction monitoring of Ser18 of Ankrd54 with phorbol 12-myristate 13-acetate stimulation

Target peptide	m/z	Charge state	Transition	Normalized quantity		Overall percentage
Target peptide	m/z	Charge state	Transition	+PMA	-PMA	+PMA	-PMA
Non-phosphorylated S18 peptide	936.078	3+	AATGGGADDESRSGRSSSDGECAVAPEPL.y4+	4.2E+02	1.9E+03	26%	91%
			AATGGGADDESRSGRSSSDGECAVAPEPL.y2+	8.5E+02	2.5E+03
			AATGGGADDESRSGRSSSDGECAVAPEPL.y15+++	2.6E+02	1.1E+03
	702.310	4+	AATGGGADDESRSGRSSSDGECAVAPEPL.y4+	ND	ND	ND	ND
	702.310	4+	AATGGGADDESRSGRSSSDGECAVAPEPL.y2+	ND	ND	ND	ND
	826.871	4+	AATGGGADDESRSGRSSSDGECAVAPEPLAEAGGL.y6+	2.0E+03	3.2E+03	61%	100%
	826.871	4+	AATGGGADDESRSGRSSSDGECAVAPEPLAEAGGL.y2+	2.0E+03	3.2E+03	61%	100%
Normalization Control (Q72-L81)	614.794	2	QQDVEPRDEL.y8+	1	1	94%	100%
			QQDVEPRDEL.y6+	1	1
			QQDVEPRDEL.y5+	1	1
Singly-phosphorylated S18 peptide	962.733	3+	AATGGGADDESRSGRSSSDGECAVAPEPL(p).y4+	1.3E+04	7.0E+03	70%	44%
			AATGGGADDESRSGRSSSDGECAVAPEPL(p).y2+	1.1E+03	1.6E+03
			AATGGGADDESRSGRSSSDGECAVAPEPL(p).y15+++
	722.302	4+	AATGGGADDESRSGRSSSDGECAVAPEPL(p).y4+	5.5E+03	2.4E+03	71%	38%
	722.302	4+	AATGGGADDESRSGRSSSDGECAVAPEPL(p).y2+	5.8E+02	9.0E+02	71%	38%
	846.862	4+	AATGGGADDESRSGRSSSDGECAVAPEPLAEAGGL(p).y6+	ND	ND	100%	83%
	846.862	4+	AATGGGADDESRSGRSSSDGECAVAPEPLAEAGGL(p).y2+	1.1E+03	8.8E+02	100%	83%

Singly-phosphorylated and nonphosphorylated forms of peptides encompassing the Ser18 residue (S18) were analysed and compared to the non-phosphorylated normalization control peptide of Ankrd54, before and after phorbol 12-myristate 13-acetate stimulation (1 µmol/L, 1 h), of immunoprecipitated Ankrd54. ND: Not detected.

Citation: Samuels AL, Louw A, Zareie R, Ingley E. Control of nuclear-cytoplasmic shuttling of Ankrd54 by PKCδ. World J Biol Chem 2017; 8(3): 163-174
URL: https://www.wjgnet.com/1949-8454/full/v8/i3/163.htm
DOI: https://dx.doi.org/10.4331/wjbc.v8.i3.163