High mobility group protein 1: A collaborator in nucleosome dynamics and estrogen-responsive gene expression

Table 1 Estrogen receptor binding affinity to consensus estrogen response element

Target	Kd (nmol/L)
N	Approximately 300
N/400 nmol/L HMGB1	52
(N’, N’’)/10 nmol/L HMGB1	50
N tailless	45
N Tailless/400 nmol/L HMGB1	Approximately 30
Restructured tailless N	Approximately 30
DNA	10

HMGB1: High mobility group protein 1.

Table 2 The effect of high mobility group protein 1 on the binding of steroid hormone receptors to their response elements

Kd (nmol/L) values
Receptor	DNA	Nucleosome	(Nucleosome/HMGB1)
ER	10	Approximately 300	Approximately 50
PR	Approximately 2	Approximately 300	Approximately 300
GR	Comparable (no specific Kd values)		No data

HMGB1: High mobility group protein 1; ER: Estrogen receptor; PR: Progesterone receptor; GR: Glucocorticoid receptor.