Recent advances in production, purification and applications of phycobiliproteins

Table 1 List of various procedures employed for phycobiliptoteins purification from cyanobacteria and red algae

PBPs	Ref.	Species	Purification procedure	Purity	Yield (%)
PC	Boussiba et al[70]	S. platensis	Ammonum sulphate precipitation; Hydroxyapatite chromatography; Ion exchange chromatography	4.15	-
APC	Reuter et al[71]	Rhodella violace	Gradient centrifugation; Hydroxylapatite chromatography; Preparative “native” PAGE	-	-
PC	Abalde et al[72]	Synechochoccus sp.	Hydrophobic interaction chromatography; Ion exchange chromatography	4.85	-
PC	Zhang et al[41]	S. platensis	Ammonum sulphate fractionation; Ion exchange chromatography; Gel filtration chromatography	5.06	-
PE	Galland-Irmouli et al[73]	Palmaria palmate	Preparative “native” PAGE	3.2	-
PC	Rito-Palomares et al[74]	S. maxima	Two-phase aqueous extraction; Ultrafiltration; Ammonum sulphate precipitation	3.8	29.5
PC	Minkova et al[75]	S. fusiformis	Rivanol treatment; Ammonum sulphate precipitation; Gel filtration chromatography; Ammonum sulphate precipitation	4.3	45.7
PC	Soni et al[39]	O. quadripunctulata	Ammonum sulphate precipitation; Gel filtration chromatography; Anion exchange chromatography	3.31	44.2
PC	Benedetti et al[76]	A. flos-aquae	Ammonum sulphate precipitation; Hydroxyapatite (electrostatic interaction) chromatography	4.78	-
PC	Patil et al[77]	S. platensis	Chitosan adsorption; Two-phase aqueous extraction	5.1	66.0
PC	Patil et al[77]	S. platensis	Chitosan adsorption; Two-phase aqueous extraction; Ion exchange chromatography	6.69	-
PC	Niu et al[78]	S. platensis	Expanded bed adsorption chromatography; Ion exchange chromatography	3.64	8.7
PC	Patil et al[42]	S. platensis	Repeated two-phase aqueous extraction; Ultrafiltration	4.05	85.0
PC	Soni et al[40]	P. fragile	Ammonum sulphate fractionation; Hydrophobic interaction chromatography	4.52	62.0
PC and APC	Yan et al[79]	S. platensis	Ammonum sulphate precipitation; Ion-exchange chromatography	5.59 and 5.19	67.04 and 80.0
PE	Sun et al[80]	Heterosiphonia japonica	Ammonum sulphate precipitation; Gel filtration chromatography; Ion exchange chromatography	4.89	-
APC	Parmar et al[81]	Geitlerinema sp. A28DM	“Ethodin” precipitation; Gel filtration chromatography	3.2	66.0
PE	Niu et al[82]	Porphyra yezoensis	Expanded bed absorption; Ion-exchange chromatography	> 4	-
APC	Su et al[83]	S. platensis	Ammonum sulphate precipitation; Hydroxyapatite chromatography; Ion-exchange chromatography	5	43.0
PE	Parmar et al[84]	Phormidium sp. A27DM, Lyngbya sp. A09DM, Halomicronema sp. A32DM	Ammonum sulphate precipitation; Gel filtration chromatography	> 3.5	> 60.0
PE	Mishra et al[85]	Pseudanabaena sp.	Ammonum sulphate precipitation; Gel filtration chromatography; Ion exchange chromatography	6.86	47.0
PC	Johnson et al[86]	Nostoc sp.	Ion exchange chromatography; Two-phase aqueous extraction	3.55	-
PE, PC and APC	Sonani et al[43]	Lyngbya sp. A09DM	Triton X-100 mediated ammonium sulphate precipitation; Ion exchange chromatography; Gel filtration chromatography	6.75, 5.53 and 5.43	76.16, 60.23 and 71.91
PE	Johnson et al[86]	Nostoc sp.	Ion exchange chromatography; Two-phase aqueous extraction	-	-

PBPs: Phycobiliproteins; PC: Phycoerythrin; APC: Allophycocyanin; PE: Phycoerythrin; PAGE: Polyacrylamide gel electrophoresis.