Discovery and characterization of the first non-coding RNA that regulates gene expression, micF RNA: A historical perspective

Figure 1 Model of two step interaction between antisense RNA I and sense RNA II-initial stem loop recognition followed by antisense/sense RNA/RNA duplex formation[34].

Figure 2 ompF mRNA levels after temperature shift from 24 °C to 42 °C. Lanes marked a-k correspond to time 0-120 min. Left, RNA from strain MC4100 that has the micF gene; right, strain SM3001 that is a micF deletion strain. Data from Andersen et al[10]. Reproduced with permission.

Figure 3 Schematic of micF gene, upstream P-10, P-35 promoter region and transcription factor binding sites. Diagram modified from Delihas et al[51].

Figure 4 Model of micF RNA/ompF mRNA interaction. Duplex based on secondary structure probing; the long range pairing of five bases at the 5’ end of ompF mRNA with positions 45-49 of micF RNA was based on computational analysis[66]. Reproduced from Delihas et al[51].

Figure 5 Multifaceted functions of micF RNA showing a “circular” regulatory process involving Lrp and micF RNA. Also shown are multiple mRNAs targeted by micF RNA. Based on work of Holmqvist et al[64] and Corcoran et al[65]. Drawing reproduced from Delihas[69].