Important relationships between Rab and MICAL proteins in endocytic trafficking

Figure 1 Schematic diagram of the MICAL protein family domain architecture. FAD: Flavin-adenine dinucleotide binding domain; CH: Calponin homology domain; LIM: LIM domain; CC: Coiled-coil; NPF: Asparagine-proline-phenylalanine motif.

Figure 2 Endogenous MICAL-L1 localized to tubular recycling endosomes. A and B: HeLa cells were grown on coverslips, fixed with 4% paraformaldehyde in PBS, immunostained with anti-MICAL-L1 antibody, followed by 568-conjugated goat anti-mouse antibody. A: MICAL-L1; B: MICAL-L1 (inset). Bar, 10 μm.

Figure 3 MICAL-L1 and MICAL-L2 regulate endocytic recycling. A: MICAL-L1 recruits Eps15 homology domain 1 (EHD1) and Rab8A to endocytic membranes and regulates recycling of transferrin and β1 integrin receptors; B: MICAL-L2/Rab13 interactions control transport of claudin-1 and occludin to the plasma membrane, whereas MICAL-L2/Rab8 complexes regulate recycling of E-cadherin. EE/SE: Early endosome/sorting endosome; RE: Recycling endosome; AEE: Apical early endosome; CRE: Common recycling endosome; ARE: Apical recycling endosome.