Fibrinogen-thrombin collagen patch reinforcement of high-risk colonic anastomoses in rats

Table 1 Adhesive score

Adhesive score
0: No adhesions
1: Extremely soft adhesions
2: Stronger adhesions, but dissectible with dull dissection
3: Stronger adhesions only dissectible with sharpen tools
4: Stenosis

Table 2 Histopathologic Biert’ scheme

Parameters	Score
	0	1	2	3
Necrosis	No	Small patches	Large patches	Massive
PMNs	Normal	Slightly increased	Strong infiltration	Massive infiltration
Lymphocytes	Normal	Slightly increased	Strong infiltration	Massive infiltration
Macrophages	Normal	Slightly increased	Strong infiltration	Massive infiltration
Edema	No	Slight	Strong	Massive
Epithelium	Glandular normal	Cubic normal	Cubic incomplete	Absent
Submucosa-muscular	Good bridges	Mild bridges	Few bridges	Bridges absent
Angiogenesis	Extensive	Strong	Slight	Absent
Fibrosis	Extensive	Strong	Slight	Absent

PMNs: Polymorphonuclear cells.

Table 3 Intra-abdominal adhesion score in survivorings animals

	Mann-Whitney mean ranges	P value
Survivors (15 d vs 30 d)	25.11 vs 11.12	0.0012
Control (15 d vs 30 d)	10.53 vs 5.5	0.017
TachoSil (15 d vs 30 d)	14.75 vs 6.25	0.001
Control vs TachoSil (15 d)	9.5 vs 10.45	0.685
Control vs TachoSil (30 d)	9.71 vs 8.50	0.584

Table 4 Most relevant parameters in histopathologic analysis (mean ± SD)

	Control	TachoSil	P value
PMNs	0.21 ± 0.42	0.78 ± 0.64	0.010
Macrophages	0.50 ± 0.65	0.89 ± 0.47	0.026
Edema	0.43 ± 0.64	0.94 ± 0.63	0.017
Epithelium regeneration	0.64 ± 0.92	1.11 ± 0.58	0.031

PMNs: Polymorphonuclear cells.