Synergistic inhibition of colorectal cancer progression by silencing Aurora A and the targeting protein for Xklp2

Table 1 Basic information on the gene expression omnibus dataset included in the study

GEO	Group	n	Stage	Tissue	Gene number
GSE21510	CN	12	0-2	Normal	715
	CRC	8	1-2b	Cancer
GSE25071	CN	3	0	Colon	788
	CRC	7	I-II	Primary tumor
GSE32323	CN	9	1-2	Normal	446
	CRC	9	1-2b	Cancer

GEO: Gene expression omnibus; CN: Control; CRC: Colorectal cancer.

Table 2 Sequence information of the primers for quantitative real time-polymerase chain reaction

Gene	Forward primer	Reverse primer
Human AURKA	5’-TGGGTGGTCAGTACATGCTC-3’	5’-TGCATCCGACCTTCAATCATTTC-3’
Human TPX2	5’-ACTTCCGCACAGATGAGCG-3’	5’-GGATGCTTTCGTAGTTCAGATGT-3’
Human β-actin	5’-GATGACCCAGATCATGTTTGAG-3’	5’-TAATGTCACGCACGATTTCC-3’

AURKA: Aurora kinase A; TPX2: Targeting protein for Xklp2.

Table 3 Antibody information

Antibody	Manufacturer	No.	Dilution ratio
AURKA antibody	Affinity	BF0123	1:2000
TPX2 antibody	Abcam	ab264124	1:5000
Cyclin B1 antibody	Affinity	AF6168	1:2000
CDK1 antibody	Abcam	ab133327	1:15000
CDK2 antibody	Affinity	AF6237	1:2000
Bax antibody	Abcam	ab182733	1:2000
Bcl-2 antibody	Affinity	AF6139	1:2000
Caspase-3 antibody	Proteintech	19677-1-AP	1:10000
p53 antibody	Affinity	AF0879	1:2000
N-cadherin antibody	Affinity	AF4039	1:2000
E-cadherin antibody	Proteintech	20874-1-AP	1:5000
Vimentin antibody	Zenbio	R22775	1:1000
p-AURKA antibody	Affinity	AF3011	1:2000
β-actin antibody	Proteintech	81115-1-RR	1:20000
Anti-rabbit IgG, HRP-linked antibody	CST	7074	1:10000

AURKA: Aurora kinase A; TPX2: Targeting protein for Xklp2; Bcl-2: B-cell lymphoma-2; Bax: B-cell lymphoma-2 associated X protein; p53: Tumor protein P53; p-AURKA: Phosphorylated Aurora kinase A; HRP: Horseradish peroxidase; CST: Cell signaling technology.

Table 4 Potential target proteins and their degrees in the relative overexpressed gene set analyzed by Cytoscape

No.	Node	Degrees
1	CCNB1	14
2	CDK1	14
3	CDKN3	13
4	UBE2C	13
5	ANLN	12
6	AURKA	12
7	CENPA	12
8	CKS2	12
9	TPX2	12
10	CENPN	11
11	TRIP13	11
12	CKAP2	10
13	CXCL10	10

Table 5 The search tool for the retrieval of interacting genes analysis results of protein-protein interaction forces (TOP 7, score = 0.999)

Node 1	Node 2	Co-expression	Experimentally determined interaction	Database annotated	Combined score
CCNB1	CDK1	0.895	0.999	0.9	0.999
AURKA	TPX2	0.846	0.988	0.9	0.999
CXCL10	CXCL11	0.824	0.994	0.9	0.999
CDK1	CKS2	0.689	0.992	0	0.999
CCNB1	CKS2	0.688	0.978	0	0.999
CENPA	CENPN	0.407	0.955	0.5	0.999
CXCL11	CXCL2	0.107	0.994	0.5	0.999