Effects of low-density lipoprotein cholesterol on lymph node metastasis after radical esophagectomy

Figure 1 The process for the retrospective study. EC: Esophageal cancer; OS: Overall survival.

Figure 2 Risk factors analysis of lymph node metastasis and survival. A: Univariate Logistic regression analysis for risk factors of lymph node metastasis; B: Multivariate Logistic regression analysis for risk factors of lymph node metastasis; C: Overall survival curves of low-density lipoprotein; D: Overall survival curves of high-density lipoprotein. LDL: Low-density lipoprotein; HDL: High-density lipoprotein; TG: Triglycerides; Apo-A1: Apolipoprotein A1; Apo-B: Apolipoprotein B.

Figure 3 Gene expression profiling interactive analysis and tumor immune estimation resource version 2 analyses based on the cancer genome atlas database. A: Expression of low-density lipoprotein receptors in esophageal cancer (EC). The levels of LRP5 and LRP6 are higher in EC than those in normal esophageal tissues; B: LRP5 and LRP6 are correlated with a poor clinical prognosis. Overall survival curves of LRP5 and LRP6; C: Correlation between LRP6 and immune cell infiltration. LRP6 is positively correlated with B lymphocyte infiltration and negatively correlated with DC infiltration.

Figure 4 Expression of LRP6 and infiltration of B lymphocytes in N+ and N0 group. A: Immunofluorescence of LRP6 and B lymphocytes in N+ and N0 group respectively; B: Quantitative analysis with Mann-Whitney U test of CD20. Infiltration of B lymphocytes are significantly higher in the N+ group; C: Quantitative analysis with Mann-Whitney U test of LRP6. Expression of LRP6 is significantly higher in the N+ group; D: Quantitative analysis with Mann-Whitney U test of Merg. Infiltration of LRP6+B lymphocytes are significantly higher in the N+ group.