Identification and differentiation of PDX1 β-cell progenitors within the human pancreatic epithelium

Table 1 Cell composition and proportion of proliferating cell nuclear antigen positive cells during pancreatic cell expansion from islet depleted pancreatic tissue

Days in culture	Percent positive
	Vimentin	EpCAM	PCNA	Vim/PCNA	EpCAM/PCNA
0 (n = 7)	22.71 ± 4.93	87.57 ± 3.96	73.57 ± 8.42	22.00 ± 5.83	74.71 ± 8.34
14 (n = 7)	80.29 ± 3.07b	23.69 ± 7.88b	88.43 ± 2.91	80.43 ± 3.24b	26.57 ± 9.75b
21 (n = 3)	99.00 ± 0.0b	3.33 ± 0.88b	99.00 ± 0.0	99.00 ± 0.0b	4.00 ± 1.53b

Data represent means ± SE. Statistical analysis of differences between the groups was performed with STATA11 (StataCorp LP, College Station, TX) using one-way analysis of variance and Bonferroni post-hoc test.

^bP < 0.01 compared to Time 0. EpCAM: Epithelial cell adhesion molecule; PCNA: Proliferating cell nuclear antigen.

Table 2 Preservation of epithelial cells during culture in a defined epithelial medium

Condition	Percent positive
	Vimentin	EpCAM
Epithelial Medium (n = 7)	44.87 ± 4.93b	24.10 ± 8.60
MSC Medium (n = 3)	95.67 ± 1.36b	17.43 ± 6.88

Data represent means ± SE. Statistical analysis of the differences between the groups was calculated with STATA11 (StataCorp LP, College Station, TX) using one-way analysis of variance and Bonferroni post-hoc test.

^bP < 0.01 for vimentin positive cells expanded in epithelial medium vs MSC medium. EpCAM: Epithelial cell adhesion molecule; MSC: Mesenchymal stem cell.