Copyright
©The Author(s) 2023.
World J Diabetes. Aug 15, 2023; 14(8): 1234-1248
Published online Aug 15, 2023. doi: 10.4239/wjd.v14.i8.1234
Published online Aug 15, 2023. doi: 10.4239/wjd.v14.i8.1234
Table 1 Sequences of miR-503 mimic/inhibitor
miRNA | Sequence (5’-3’) |
miR-503 mimic | TAGCAGCGGGAACAGTACTGCAG |
miR-503 mimic NC | GACAGAGACACGAGCGGCTGTAT |
miR-503 inhibitor | CTGCAGTACTGTTCCCGCTGCTA |
miR-503 inhibitor NC | TAGTAACCGTCTTTCCCCTGGGC |
Table 2 Reverse transcription-quantitative polymerase chain reaction primers
Primer | Sequence (5’-3’) |
miR-151-3p-F | ACACTCCAGCTGGGCUAGACUGAGGCUCC |
miR-151-3p-R | CTCAACTGGTGTCGTGGA |
miR-19b-3p-F | ACACTCCAGCTGGGUGUGCAAAUCCAUGCAA |
miR-19b-3p-R | CTCAACTGGTGTCGTGGA |
miR-106b-5p-F | ACACTCCAGCTGGGUAAAGUGCUGACAGU |
miR-106b-5p-R | CTCAACTGGTGTCGTGGA |
miR-30e-5p-F | ACACTCCAGCTGGGUGUAAACAUCCUUGAC |
miR-30e-5p-R | CTCAACTGGTGTCGTGGA |
miR-122-5p-F | ACACTCCAGCTGGGUGGAGUGUGACAAUGG |
miR-122-5p-R | CTCAACTGGTGTCGTGGA |
miR-29a-3p-F | ACACTCCAGCTGGGUAGCACCAUCUGAAAU |
miR-29a-3p-R | CTCAACTGGTGTCGTGGA |
miR-29c-3p-F | ACACTCCAGCTGGGUAGCACCAUUUGAAAU |
miR-29c-3p-R | CTCAACTGGTGTCGTGGA |
miR-497-5p-F | ACACTCCAGCTGGGCAGCAGCACACUGUGG |
miR-497-5p-R | CTCAACTGGTGTCGTGGA |
miR-101a-3p-F | ACACTCCAGCTGGGUACAGUACUGUGAUA |
miR-101a-3p-R | CTCAACTGGTGTCGTGGA |
miR-741-3p-F | ACACTCCAGCTGGGAAAGAUGCCACGCUAU |
miR-741-3p-R | CTCAACTGGTGTCGTGGA |
miR-503-F | ACACTCCAGCTGGGTAGCAGCGGGAACAGTA |
miR-503-R | CTCAACTGGTGTCGTGGA |
miR-671-F | ACACTCCAGCTGGGUCCGGUUCUCAGGGC |
miR-671-R | CTCAACTGGTGTCGTGGA |
U6-F | CTCGCTTCGGCAGCACA |
U6-R | AACGCTTCACGAATTTGCGT |
SIRT4-F | TCCTGGGAGTGGACAGAATGA |
SIRT4-R | CTGTGGATCCATGGGAACGC |
Caspase 12-F | TGCCAATTCCGACAAACAGC |
Caspase 12-R | CTGGATTCCCTGAGGAACTGT |
GRP78-F | AACCCAGATGAGGCTGTAGCA |
GRP78-R | ACATCAAGCAGAACCAGGTCAC |
CHOP-F | CCAGCAGAGGTCACAAGCAC |
CHOP-R | CGCACTGACCACTCTGTTTC |
β-actin-F | CACCCGCGAGTACAACCTTC |
β-actin-R | CCCATACCCACCATCACACC |
Table 3 Detection of 12 differentially expressed miRNAs between diabetes mellitus rats and normal rats by miRNA sequencing
miRNA_id | Log2 FC | P value |
rno-miR-151-3p | > 1.0 | < 0.001 |
rno-miR-19b-3p | < -1.0 | < 0.001 |
rno-miR-106b-5p | < -1.0 | < 0.001 |
rno-miR-30e-5p | < -1.0 | < 0.001 |
rno-miR-122-5p | > 1.0 | < 0.001 |
rno-miR-29a-3p | < -1.0 | < 0.001 |
rno-miR-29c-3p | < -1.0 | < 0.001 |
rno-miR-497-5p | < -1.0 | < 0.001 |
rno-miR-101a-3p | < -1.0 | < 0.001 |
rno-miR-741-3p | > 1.0 | < 0.001 |
rno-miR-503 | < -1.0 | < 0.001 |
rno-miR-671 | > 1.0 | < 0.001 |
Table 4 Identification of mRNAs that may bind to miR-503 by sequencing results combined with the miRDB database
No. | Gene_id |
1 | SLC12A1 |
2 | SIRT4 |
3 | MLYCD |
4 | FBXL22 |
5 | CCND1 |
6 | MAP2K1 |
7 | PCP4 |
8 | RPS6KA3 |
9 | EMC6 |
10 | PPFIA3 |
11 | TTC17 |
12 | RAB11FIP1 |
13 | GALNT2 |
14 | NCS1 |
15 | APLN |
16 | CEP85L |
17 | CLSTN1 |
18 | KIF1C |
19 | PIGQ |
20 | BTRC |
21 | LOC691995 |
22 | EGLN3 |
23 | NABP1 |
- Citation: Su BL, Wang LL, Zhang LY, Zhang S, Li Q, Chen GY. Potential role of microRNA-503 in Icariin-mediated prevention of high glucose-induced endoplasmic reticulum stress. World J Diabetes 2023; 14(8): 1234-1248
- URL: https://www.wjgnet.com/1948-9358/full/v14/i8/1234.htm
- DOI: https://dx.doi.org/10.4239/wjd.v14.i8.1234