Polymorphisms in HIF-1a gene are not associated with diabetic retinopathy in China

Table 1 Baseline characteristics of the study subjects

Group	Total	Male (%)	Female (%)	Mean age (yr)
Diabetes	150	66	84	55.84 ± 3.66
Diabetic retinopathy	149	62	87	55.97 ± 4.66
Controls	144	67	77	56.32 ± 4.70
				P = 0.84

Table 2 Hardy-Weinberg analysis of rs11549467 and rs11549465

Group	SNP loci	Genotype	Actual cases	Examples of H-W equilibrium theory	P value
Diabetes	11549467	GG|GA|AA	142|8|0	142.11|7.79|0.11	0.7372
	11549465	CC|CT|TT	135|15|0	135.38|14.25|0.38	0.5192
Diabetic retinopathy	11549467	GG|GA|AA	140|9|0	140.14|8.73|0.14	0.7038
	11549465	CC|CT|TT	130|19|0	130.61|17.79|0.61	0.4058
Controls	11549467	GG|GA|AA	137|7|0	137.09|6.83|0.09	0.7650
	11549465	CC|CT|TT	125|19|0	125.63|17.75|0.63	0.3967

SNP: Single nucleotide polymorphisms.

Table 3 Distribution of the genotype and allele frequencies of rs11549467 and rs115469465 in the diabetes, diabetic retinopathy and control group

SNP	Patient/control	Genotype, n (%)			Allele, n (%)
rs11549467		G/G	G/A	A/A	G	A
	Diabetes	142 (94.67)	8 (5.33)	0 (0)	292 (97.33)	8 (2.67)
	Diabetic retinopathy	140 (93.96)	9 (6.04)	0 (0)	289 (96.98)	9 (3.02)
	Controls	137 (95.14)	7 (4.86)	0 (0)	281 (97.57)	7 (2.43)
						P > 0.05
rs11549465		C/C	C/T	T/T	C	T
	Diabetes	135 (90)	15 (10)	0 (0)	285 (95)	15 (5)
	Diabetic rentinopathy	130 (87.25)	19 (12.75)	0 (0)	279 (93.62)	19 (6.38)
	Controls	125 (86.81)	19 (13.19)	0 (0)	269 (93.4)	19 (6.6)
						P > 0.05

SNP: Single nucleotide polymorphisms.

Table 4 Haplotype association analysis

rs11549467	rs11549465	Diabetes frequency	Diabetic retinopathy frequency	Control frequency	P value
G	C	92.535	90.604	90.972	-
G	T	4.798	6.376	6.597	0.13
A	C	2.465	3.020	2.431	0.29