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©The Author(s) 2023.
World J Gastrointest Oncol. Sep 15, 2023; 15(9): 1531-1543
Published online Sep 15, 2023. doi: 10.4251/wjgo.v15.i9.1531
Published online Sep 15, 2023. doi: 10.4251/wjgo.v15.i9.1531
Figure 3 The RNA-binding protein cleavage and polyadenylation factor 6 binds to the 3’-untranslated region of integrin binding sialoprotein and regulates its expression.
A: Potential RNA-binding proteins that can bind to integrin binding sialoprotein (IBSP) were analyzed through bioinformatic analysis; B: The levels of cleavage and polyadenylation factor 6 (CPSF6) in stomach adenocarcinoma; C: The mRNA expression of CPSF6 was detected in GC tissues and normal adjacent tissues by real-time quantitative polymerase chain reaction (RT-qPCR); D: The correlation between CPSF6 and IBSP was verified; E and F: The binding ability between CPSF6 and IBSP was confirmed by luciferase reporter and RNA immunoprecipitation chip assays; G and H: The mRNA and protein expression of CPSF6 and IBSP was measured in the si-NC, si-CPSF6#1, and si-CPSF6#2 groups by RT-qPCR and Western blot, respectively. aP < 0.05, bP < 0.01.
- Citation: Wang XJ, Liu Y, Ke B, Zhang L, Liang H. RNA-binding protein CPSF6 regulates IBSP to affect pyroptosis in gastric cancer. World J Gastrointest Oncol 2023; 15(9): 1531-1543
- URL: https://www.wjgnet.com/1948-5204/full/v15/i9/1531.htm
- DOI: https://dx.doi.org/10.4251/wjgo.v15.i9.1531