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©The Author(s) 2023.
World J Gastrointest Oncol. Sep 15, 2023; 15(9): 1531-1543
Published online Sep 15, 2023. doi: 10.4251/wjgo.v15.i9.1531
Published online Sep 15, 2023. doi: 10.4251/wjgo.v15.i9.1531
Figure 2 Integrin binding sialoprotein downregulation suppresses cell proliferation, migration, and invasion and facilitates pyroptosis.
A and B: The mRNA and protein expression levels of integrin binding sialoprotein (IBSP) were assessed in the si-NC, si-IBSP#1, and si-IBSP#2 groups by real-time quantitative polymerase chain reaction and Western blot in MKN45 and HGC-27 cells, respectively; C: Cell viability was detected after silencing IBSP by Cell-Counting Kit-8 assay in MKN45 and HGC-27 cells; D and E: Cell invasion and migration were evaluated by Transwell assay in MKN45 and HGC-27 cells; F: Pyroptosis was measured after IBSP knockdown by flow cytometry in MKN45 and HGC-27 cells; G: The protein expression levels of NLR family pyrin domain containing 3, cleaved caspase-1, interleukin-18 (IL-18), and IL-1β were examined by Western blot after suppressing IBSP in MKN45 and HGC-27 cells. bP < 0.01. IBSP: Integrin binding sialoprotein; IL: Interleukin; NLRP3: NLR family pyrin domain containing 3.
- Citation: Wang XJ, Liu Y, Ke B, Zhang L, Liang H. RNA-binding protein CPSF6 regulates IBSP to affect pyroptosis in gastric cancer. World J Gastrointest Oncol 2023; 15(9): 1531-1543
- URL: https://www.wjgnet.com/1948-5204/full/v15/i9/1531.htm
- DOI: https://dx.doi.org/10.4251/wjgo.v15.i9.1531