Habashy DA, El Tayebi HM, Fawzy IO, Hosny KA, Esmat G, Abdelaziz AI. Interplay between microRNA-17-5p, insulin-like growth factor-II through binding protein-3 in hepatocellular carcinoma. World J Hepatol 2016; 8(23): 976-984 [PMID: 27621763 DOI: 10.4254/wjh.v8.i23.976]
Corresponding Author of This Article
Ahmed Ihab Abdelaziz, MD, PhD, Associate Professor of Molecular Medicine, Department of Biology, American University in Cairo, New Cairo City, AUC Avenue, Cairo 11835, Egypt. ahmed.ihab.abdelaziz@gmail.com
Research Domain of This Article
Oncology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Hepatol. Aug 18, 2016; 8(23): 976-984 Published online Aug 18, 2016. doi: 10.4254/wjh.v8.i23.976
Table 1 Characteristic features of non-metastatic hepatocellular carcinoma patients and healthy controls
Average ± SD
HCC and cirrhotic patient parameters
Mean age
49 ± 13.5
Sex: Male/female
22/1
Ethanol abuse
None
AST (U/L)
100.5 ± 65.8
ALT (U/L)
85.6 ± 95.6
Alkaline phosphatase (U/L)
110.2 ± 60.7
Serum albumin (g/dL)
4.6 ± 1.5
Serum AFP (ng/mL)
155.7 ± 22.3
HCV Ab
100% (23/23 HCC patients)
HBV Ab
17.3% (4/23 HCC patients)
Healthy control (liver donor) parameters
Mean age
31 ± 10.5
Sex: Male/female
7/3
Ethanol abuse
None
HCV Ab
None
HBV Ab
None
Table 2 Number/sizes of focal lesions according to Milan criteria
Patients
No. of focal lesions
Size of focal lesions (cm)
Patient 1
3 focal lesions
1.5, 1 and 1
Patient 2
Unifocal
2.5
Patient 3
3 focal lesions
2, 2.5 and 3
Patient 4
3 focal lesions
2, 2 and 3.5
Patient 5
Unifocal
1.5-2
Patient 6
3 focal lesions
3-4, 1 and 1
Patient 7
Unifocal
4
Patient 8
3 focal lesions
4, 1 and 1
Patient 9
3 focal lesions
1, 1 and 1.5
Patient 10
Unifocal
2.5
Patient 11
2 focal lesions
1 and 1.7
Patient 12
3 focal lesions
1, 1 and 1
Patient 13
Unifocal
3
Patient 14
3 focal lesions
3, 1.5 and 2
Patient 15
3 focal lesions
1, 1 and 4
Patient 16
2 focal lesions
3 and 1.5
Patient 17
2 focal lesions
1.5 and 3
Patient 18
3 focal lesions
2.5, 2.5 and 1.5
Patient 19
3 focal lesions
1.5, 1 and 1
Patient 20
Unifocal
2
Patient 21
Unifocal
1.5
Patient 22
3 focal lesion
3, 2.5 and 1
Patient 23
Unifocal
3
Table 3 The forward and reverse primer sequences used in the wild type 1 and 2, and the mutant type 1 and 2 insulin-like growth factor binding protein-3 3’UTR constructs
Citation: Habashy DA, El Tayebi HM, Fawzy IO, Hosny KA, Esmat G, Abdelaziz AI. Interplay between microRNA-17-5p, insulin-like growth factor-II through binding protein-3 in hepatocellular carcinoma. World J Hepatol 2016; 8(23): 976-984