CYP2E1 immunoglobulin G4 subclass antibodies after desflurane anesthesia

Table 1 Characteristics of the study population (n = 39)

Demographic characteristics	Mean ± SD/median (IQR) or frequencies1	Range2
Age (yr)	42 (29-62)	20-75
Gender (M/F)	22(56%) 17(44%)	-
Height (cm)	172.62 (10.55)	152-192
Weight (Kg)	82.7 (20.55)	35-140
BMI (Kg/m2)	27.66 (6.14)	12.85-41.91
ASA I/II/III	16 (41%)/22 (56%)/1 (3%)	-
Anesthetic data
Previous anesthesias (no/yes)	12 (31%)/27 (69%)	-
No of previous anesthesias	1 (0-2)	0-6
Duration of anesthesia (min)	150 (90-180)	30-360
Length of stay (d)	5 (4-7)	2-15
Type of operation (n)	Orthopedic: 35
	Fractures: 18
	Arthroscopies: 12
	Knee/hip arthroplasties: 5
	Thyroidectomies (non-malignant): 3
	Saphenectomy: 1

¹Data are presented as mean ± SD or as median [interquartile range (IQR)] according to normality of continuous variables;

²Qualitative variables are presented as absolute and relative frequencies.

Table 2 Comparison of blood test measurements before (baseline) and 96 h after exposure to desflurane

	Baseline measurements	Measurements after intervention	P value1
Blood count
HCT (%)	41.2 (37.4-43)	36.75 (31.65-41.7)	0.016
HBG (g/dL)	13.56 (1.944)	12.01 (1.941)	0.002
WBC	7907.94 (2218.9)	8330 (1826.7)	0.446
PLT (x 103)	251 (217-284)	241 (205-276)	0.259
Liver function tests
SGOT (IU/L)	20 (17-25)	21.5 (17-28)	0.489
SGPT (IU/L)	19 (14-39)	19.5 (13-30)	0.987
ALP (IU/L)	64 (46-77)	54 (47-63)	0.222
γGT (IU/L)	22.5 (13-35)	23 (13-30)	0.532
Direct bilirubin (mg/dL)	0.2 (0.13-0.21)	0.185 (0.1-0.2)	0.277
Indirect bilirubin (mg/dL)	0.5 (0.3-0.7)	0.4 (0.3-0.6)	0.840
Total bilirubin (mg/dL)	0.6 (0.5-1)	0.6 (0.4-0.7)	0.610
Proteins (g/dL)	6.3 (6-6.7)	5.9 (5.35-6.45)	0.082
Albumin (g/dL)	4.1 (3.7-4.3)	3.5 (3.15-3.95)	0.013
PT (s)	11.9 (11.23-13)	12.55 (11.6-13.5)	0.219
aPTT (s)	27.97 (3.09)	29.83 (5.09)	0.125
INR	0.97 (0.92-1.08)	1.02 (0.9-1.1)	0.346
IgG4 (g/L)	0.04 (0.02 -0.5)	0.065 (0.02-0.415)	0.576
αGST (ng/mL)	3.5 (1.69-4.38)	3.03 (1.11-4.87)	0.489

¹Statistical tests applied: Student’s t test, Mann-Whitney U test. Data are presented as the mean ± SD or as the median [interquartile range (IQR)] according to normality of continuous variables. SGOT: Aspartate aminotransferase; SGPT: Alanine aminotransferase; ALP: Alkaline phosphatase; GT: Glutamyl-transpeptidase; PT: Prothrombin time; aPTT: Activated partial thromboplastin time; INR: International normalized ratio; αGST: Alpha-glutathione-S-transferase.

Table 3 Multivariate linear regression model of the mean difference of alpha-glutathione-S-transferase adjusted for gender, body mass index, duration of anesthesia, number of times anesthesia was administered previously, and length of stay

αGST	Coefficient	Standard error	P value	95%CI
Gender	2.15	1.02	0.0491	0.0111-4.284
Length of stay	-0.445	0.202	0.041	-0.868-(-0.0229)
Duration	0.0323	0.008	0.0011	0.0157-0.0489

¹Statistical tests applied: Student’s t test, Mann-Whitney U test. Data are presented as the mean ± SD or as the median (IQR) according to normality of continuous variables. αGST: Alpha-glutathione-S-transferase.

Table 4 Comparisons of IgG4 and alpha-glutathione-S-transferase levels according to medical history of receiving or not receiving anesthesia previously

	No previous anesthesia (n = 12)	Previous anesthesia (n = 27)	P value1
IgG4 levels
Baseline	0.025 (0-0.04)	0.08 (0.02-0.66)	0.099
96 h after anesthesia	0.035 (0-0.07)	0.105 (0.03-0.74)	0.051
αGST levels
Baseline	2.94 (1.69-3.94)	3.54 (1.42-5.87)	0.505
96 h after anesthesia	3.825 (3.02-5.4)	2.65 (1.05-4.33)	0.147

¹Mann-Whitney U test. Data are presented as the median interquartile range; αGST: Alpha-glutathione-S-transferase.