Prevalence of HIV and HCV infections in two populations of Malian women and serological assays performances

Table 1 Hepatitis C virus serological and biomolecular profile of 2 positive pregnant women

Subject	MONOLISA-HCV			INNOTEST-HCV			LIA-HCV						LIA-HCV	PCR HCV	Genotype HCV
	(test ratio value)			(test ratio value)			(reactivity score)
	X1	X2	X3	X1	X2	X3	C1	C2	E2	NS3	NS4	NS5
AD3	5.84	6.12	6.08	5.50	5.71	5.58	4	1	0	3	2	0	Pos	Pos	2
OM4	0.84	0.28	0.27	3.32	2.54	2.38	1	0	0	0	1	0	Pos	Neg	-

AD3 and OM4: Initials of pregnant women from reference health center (municipality No. 3 and No. 4); X1: Test ratio value for initial essay; X2, X3: Test ratio value for replicate assays; C1 and C2: Core protein 1 and 2 of hepatitis C virus (HCV); E2: Transmembrane protein of HCV; NS3, NS4 and NS5: Nonstructural proteins of HCV. PCR: Polymerase chain reaction.

Table 2 Human immunodeficiency virus and hepatitis C virus seroprevalences, hepatitis C virus-RNA and genotype in the 2 series

Series	n	HIV Ab	HCV Ab	PCR-HCV	HCV genotype
		%	%	%	1	2	other
Series 1	1000	4.10	0.20	0.10	0	1	0
W ≤ 50 yr old
Series 2	231	6.06	6.49	5.60	2	10	0
W > 50 yr old

Series 1 (W ≤ 50 years old): Young women population; Series 2 (W > 50 years old): Older women; HIV Ab: Serological markers of human immunodeficiency virus (HIV) (or anti-HIV); HCV Ab: Serological markers of hepatitis C virus (HCV) (or anti-HCV); PCR HCV: Virological markers of HCV [polymerase chain reaction (PCR) reactive results, or RNA detection]; %: Prevalence.

Table 3 Predictive values of the Monolisa test ratio in both series of samples

MONOLISA HCV-Ab Ag	Series	N+	LIA-HCV			PPV, %	PCR-RNA-HCV			PPV, %
Test ratio			Neg	Pos	Ind		Neg	Pos	Ind
(1-3)	1	10	6	0	4	0	6	0	41	0
(> 3)	1	7	1	1	5	50	6	1	0	14.3
(1-3)	2	7	2	1	4	33.3	7	0	0	0
(> 3)	2	14	0	14	0	100	1	13	0	92.9
(1-3)	1 + 2	17	8	1	8	11.1	13	0	41	0
(> 3)	1 + 2	21	1	15	5	93.8	7	14	0	66.7

¹Four samples [whose test ratio = (1-3)] were not tested with polymerase chain reaction (PCR) because Monolisa results were not reproducible and the Innotest was negative in each case. Ind: Indeterminate result; PPV: Positive predictive value of hepatitis C virus (HCV) Monolisa test for antibody or RNA detection; Series 1: Young women; Series 2: Women over 50 years old; Series (1 + 2): Overall cohort (young and older women); N+: Numbers of positive results with Monolisa HCV according to the test ratio value.

Table 4 LIA profiles and polymerase chain reaction results

No.	HCV LIA profile (bands’ intensity)						PCR HCV	Viral load (IU/mL)
	C1	C2	E2	NS3	NS4	NS5
1	4	3	0	4	1	0	pos	32 350
2	3	1	0	4	0	0	pos	1 442 298
3	1	1	0	0.5	0	0	neg	Not detected
4	4	2	0	4	0	0	pos	2 701 467
5	4	3	0	4	0.5	0.5	pos	3 247 624
6	0	0	0	4	0	0	pos	290 103
7	4	2	0	4	3	0	pos	98 440
8	4	4	0	4	0	0	pos	20 503
9	3	2	1	4	0	0	pos	683 959
10	3	1	0	2	0	0	neg	Not detected
11	3	1	0	4	0	2	pos	6 369 090
12	4	2	0	4	1	3	pos	831 422
13	4	3	2	4	0	0	pos	12 352 743
14	3	1	0	4	0.5	0	pos	4 019 864
15	2	3	4	4	4	0	pos	< 12
16	4	1	0	3	2	0	pos	1600
17	1	0	0	0	1	0	neg	Not detected
BP	16	15	3	16	8	3

C1 and C2: Core protein 1 and 2 for hepatitis C virus (HCV); E2: Transmembrane protein of HCV; NS3, NS4 and NS5: Nonstructural proteins of HCV. PCR: Polymerase chain reaction; BP: Band presence.