Construction of non-covalent single-chain Fv dimers for hepatocellular carcinoma and their biological functions

Table 1 Primer sequence

	scFv-5 residues
VH	Sense: 5’-TATGGCCCAGCCGGCCATGG-3’
	Antisense: 5’-AGAACCACCACCACCTGAGGAGACGGTGACCGT-3’
VL	Sense: 5’-TCAGGTGGTGGTGGTTCTGACATTGAGCTCACCCAGTCTCCA-3’
	Antisense: 5’-TATGCGGCCGCCCGTTTCA-3’
	scFv-4 residues
VH	Sense: 5’-TATGGCCCAGCCGGCCATGG-3’
	Antisense: 5’-AGAACCACCACCTGAGGAGACGGTGACCGT-3’
VL	Sense: 5’-TCCTCAGGTGGTGGTTCTGACATTGAGCTCACCCAGTCTCCA-3’
	Antisense: 5’-TATGCGGCCGCCCGTTTCA-3’
	scFv-3 residues
VH	Sense: 5’-TATGGCCCAGCCGGCCATGG-3’
	Antisense: 5’-AGAACCACCTGAGGAGACGGTGACCGT-3’
VL	Sense: 5’-GTCTCCTCAGGTGGTTCTGACATTGAGCTCACCCAGTCTCCA-3’
	Antisense: 5’-TATGCGGCCGCCCGTTTCA-3’
	Sequencing primers
	Sense: 5’-CAA CGT GAA AAA ATTATT CGC-3’
	Antisense: 5’-GTA AAT GAA TTT TCT GTA TGA GG-3’