Case Control Study
Copyright ©The Author(s) 2021.
World J Hepatol. May 27, 2021; 13(5): 557-570
Published online May 27, 2021. doi: 10.4254/wjh.v13.i5.557
Table 1 Comparison of the gut microbiome at different taxonomic levels between the groups
Taxa
Heath controls (n = 21)
Cirrhosis with non-severe dysbiosis (n = 24)
Cirrhosis with severe dysbiosis (n = 24)
P value, Non-severe dysbiosis vs controls
P value, Severe dysbiosis vs controls
P value, Severe dysbiosis vs non-severe one
Firmicutes91.8 (89.3-96.4)89.7 (73.0-93.6)80.1 (62.7-88.1)0.074< 0.0010.028
Clostridia88.0 (86.6-91.7)83.5 (69.8-88.7)69.8 (57.4-77.2)0.008< 0.0010.001
Ruminococcaceae33.9 (28.1-41.6)27.6 (19.2-36.5)18.8 (7.9-31.7)0.0860.0020.081
Lachnospiraceae43.8 (37.2-54.6)37.6 (27.2-60.5)31.0 (22.1-46.0)0.4880.0300.190
Bacilli0.1 (0.0-0.2)0.5 (0.2-1.9)7.1 (1.3-14.8)< 0.001< 0.001< 0.001
Streptococcaceae0.03 (0.02-0.10)0.29 (0.12-0.52)3.20 (0.38-10.4)< 0.001< 0.0010.002
Lactobacillaceae0.00 (0.00-0.01)0.02 (0.01-0.22)0.47 (0.12-1.50)0.002< 0.001< 0.001
Enterococcaceae0.00 (0.00-0.00)0.00 (0.00-0.03)0.03 (0.01-0.08)0.0670.001< 0.001
Bacteroidetes5.6 (2.8-8.1)5.7 (1.8-12.9)6.1 (3.2-8.2)0.9540.8290.959
Bacteroidaceae2.5 (0.8-3.4)1.3 (0.6-4.3)1.4 (0.2-3.8)0.9910.4320.261
Actinobacteria0.2 (0.1-0.3)0.8 (0.3-2.8)0.7 (0.4-2.9)< 0.001< 0.0010.687
Bifidobacteriaceae0.1 (0.0-0.2)0.6 (0.1-2.6)0.5 (0.2-2.3)0.0020.0010.687
Proteobacteria0.39 (0.14-0.51)0.15 (0.10-0.81)3.57 (1.77-6.65)0.869< 0.001< 0.001
Enterobacteriaceae0.03 (0.01-0.05)0.04 (0.02-0.61)2.70 (1.58-6.24)0.104< 0.001< 0.001
Table 2 Main indicators of patients with cirrhosis with severe and non-severe dysbiosis

Severe dysbiosis (n = 24)
Non-severe dysbiosis (n = 24)
P value
Age, yr51.5 (42.0-59.0)50.0 (35.0-57.5)0.392
Body mass index, kg/m224.6 (22.8-27.7)24.2 (22.7-27.7)0.837
Male/female12/1211/130.500
Etiology of cirrhosis: Alcohol990.617
Autoimmune270.068
HBV720.068
HCV530.350
Cryptogenic130.304
Child–Turcotte–Pugh score9 (8-10)7 (6-9)0.047
Death1330.001
Death within the first year of follow-up520.092
Death during the following years of follow-up810.002
Esophageal varices (present/absent)20/418/60.477
Hepatic encephalopathy (overt/minimal/absent)11/9/46/11/70.288
Number connection test, seconds87 (65-118)79 (59-92)0.248
Ascites (present/absent)16/811/130.122
Spontaneous bacterial peritonitis (present/absent)0/240/241.000
Red blood cells, 1012 cell/L3.8 (3.4-4.0)3.9 (3.6-4.5)0.370
White blood cells, 109 cell/L3.8 (2.7-5.3)3.8 (3.1-5.2)0.628
Platelets, 109 cell/L87 (55-120)76 (60-108)0.860
Serum total protein, g/L70 (61-76)73 (64-78)0.599
Serum albumin, g/L31 (28-37)38 (34-41)0.009
Serum total bilirubin, μmol/L47 (31-62)31 (24-63)0.375
Prothrombin index (Quick test), %58 (48-67)64 (54-71)0.239
Creatinine, mg/dL0.69 (0.53-0.87)0.73 (0.66-0.90)0.187
Serum sodium, mmol/L141 (139-144)141 (138-143)0.795
Serum potassium, mmol/L4.3 (4.0-4.7)4.3 (4.1-4.7)0.844
Serum glucose, mmol/L5.1 (4.7-5.6)5.3 (4.7-6.0)0.260
Alanine aminotransferase, U/L36 (25-72)37 (23-60)0.804
Aspartate aminotransferase, U/L54 (41-98)40 (26-67)0.219
Gamma glutamyl transferase, U/L77 (40-148)76 (36-131)0.621
Alkaline phosphatase, U/L221 (188-340)222 (166-298)0.542
Cholinesterase, kU/L2.7 (1.9-3.7)4.0 (3.6-4.5)0.031
C-reactive protein, mg/L10.1 (2.1-16.1)2.1 (0.3-8.9)0.032
Splenic length, cm15.4 (14.0-17.6)16.1 (13.3-19.2)0.841