Copyright
©The Author(s) 2021.
World J Hepatol. Mar 27, 2021; 13(3): 328-342
Published online Mar 27, 2021. doi: 10.4254/wjh.v13.i3.328
Published online Mar 27, 2021. doi: 10.4254/wjh.v13.i3.328
Table 1 Expression rate of hepatocytes positive for proliferating cell nuclear antigen was calculated as number of positive field expression in 10 fields per rat liver tissue
Group | 10 wk duration | 16 wk duration |
DEN | + | ++ |
DEN + 100 AAF | + | ++ |
DEN + 200 AAF | ++ | +++ |
DEN + 300 AAF | ++ | +++ |
Table 2 Effect of diethylnitrosamine and 2-acetylaminofluorene on alpha-fetoprotein and liver function
AFP | ALT | Total bilirubin | Direct bilirubin | Albumin | |
Naïve | 22.8 ± 1.13 | 33.3 ± 6.83 | 0.30 ± 0.18 | 0.27 ± 0.14 | 3.77 ± 0.23 |
Week 10 | |||||
DEN | 89.2 ± 28.8d | 63.0 ± 27.5d | 1.44 ± 0.45d | 1.03 ± 0.14d | 2.49 ± 0.15d |
DEN + 100 AAF | 116 ± 52.1d | 78.3 ± 17.8d | 2.07 ± 0.44d,e | 1.40 ± 0.39d | 2.83 ± 0.19d |
DEN + 200 AAF | 223 ± 124b,d | 82.7 ± 12.7d | 2.73 ± 0.23a,d,e | 1.67 ± 0.19a,d,e | 2.73 ± 0.14d |
DEN + 300 AAF | 305 ± 126d,e | 98.0 ± 10.7d,e | 3.13 ± 0.36b,d,e | 2.13 ± 0.61d,e | 3.15 ± 0.38c,d,e |
Week 16 | |||||
DEN | 159 ± 32.2d | 94.1 ± 6.4d | 2.13 ± 0.55d | 1.60 ± 0.39d | 2.0 ± 0.62d |
DEN + 100 AAF | 290 ± 241d | 104 ± 31.9d | 2.23 ± 0.36d | 2.25 ± 0.63d | 2.57 ± 0.37d |
DEN + 200 AAF | 815 ± 143a,d,f | 128 ± 36.9d | 4.10 ± 0.39a,d,f | 2.53 ± 0.63a,d,f | 2.17 ± 0.29d |
DEN + 300 AAF | 1059 ± 360b,d,f | 210 ± 63.2b,c,d,f | 4.47 ± 0.99b,d,f | 3.10 ± 0.39d,f | 2.13 ± 0.67b,c,d,f |
Table 3 Effect of diethylnitrosamine and 2-acetylaminofluorene on relative quantification of lncRNA-RP11-513I15.6 (long-noncoding RNA), relative quantification of miR-1262 and relative quantification of miR-1298 (microRNA)
RQ of lncRNA-RP11-513I15.6 | RQ of miR-1262 | RQ of miR-1298 | ||||
Liver | Serum | Liver | Serum | Liver | Serum | |
Naïve | 2.33 ± 0.31 | 1.86 ± 0.41 | 0.38 ± 0.09 | 0.26 ± 0.34 | 0.77 ± 0.26 | 0.1 ± 0.04 |
Week 10 | ||||||
DEN | 1.31 ± 0.36d | 0.99 ± 0.15d | 1.63 ± 0.28d | 1.40 ± 0.13d | 1.55 ± 0.37d | 1.08 ± 0.11d |
DEN + 100 AAF | 1.03 ± 0.2d | 0.63 ± 0.13d,e | 2.26 ± 0.54d | 2.23 ± 0.19d | 1.85 ± 0.12d | 1.48 ± 0.56d |
DEN + 200 AAF | 0.58 ± 0.12a,d,e | 0.44 ± 0.05d,e | 2.52 ± 0.44d,e | 2.81 ± 0.49d,e | 2.46 ± 0.37d,e | 1.82 ± 0.47b,c,d,e |
DEN + 300 AAF | 0.47 ± 0.037b,d,e | 0.39 ± 0.005d,e | 3.9 ± 0.36d,e | 3.59 ± 1.10b,d,e | 2.88 ± 0.11d,e | 3.30 ± 0.18d,e |
Week 16 | ||||||
DEN | 0.76 ± 0.1d | 0.52 ± 0.13d | 3.12 ± 0.62d | 2.15 ± 0.08d | 2.52 ± 0.56d | 2.23 ± 0.26d |
DEN + 100 AAF | 0.46 ± 0.04d,f | 0.22 ± 0.04d | 4.48 ± 0.63d,f | 4.08 ± 0.32d | 3.92 ± 0.61d,f | 4.56 ± 0.61d,f |
DEN + 200 AAF | 0.26 ± 0.07d,f | 0.14 ± 0.07d,f | 5.71 ± 0.76a,d,f | 7.38 ± 2.24d,f | 4.58 ± 0.56d,f | 5.78 ± 1.72d,f |
DEN + 300 AAF | 0.14 ± 0.04b,d,f | 0.12 ± 0.06d,f | 6.45 ± 1.04b,d,f | 9.78 ± 4.32b,d,f | 5.89 ± 1.27d,f | 7.38 ± 2.05b,d,f |
- Citation: Hasanin AH, Habib EK, El Gayar N, Matboli M. Promotive action of 2-acetylaminofluorene on hepatic precancerous lesions initiated by diethylnitrosamine in rats: Molecular study. World J Hepatol 2021; 13(3): 328-342
- URL: https://www.wjgnet.com/1948-5182/full/v13/i3/328.htm
- DOI: https://dx.doi.org/10.4254/wjh.v13.i3.328