Copyright
©The Author(s) 2021.
World J Hepatol. Dec 27, 2021; 13(12): 2052-2070
Published online Dec 27, 2021. doi: 10.4254/wjh.v13.i12.2052
Published online Dec 27, 2021. doi: 10.4254/wjh.v13.i12.2052
Table 1 Atherogenic ratios, inflammation and endothelial dysfunction markers in a nutritional model of steatohepatitis
Variable | Control (n = 10) | Intervention (n = 10) | P value |
AC | 0.6 (0.2–0.9) | 2.5 (1.5–3.4) | < 0.001a |
CRI-I | 1.6 (± 0.4) | 3.5 (± 1.1) | < 0.001a |
CRI-II | 0.3 (± 0.1) | 0.8 (± 0.2) | < 0.001a |
IL-1β (pg/mL) | 367.7 (± 31.2) | 465.9 (± 52.7) | 0.001a |
MCP-1 (ng/mL) | 2.7 (± 0.6) | 3.8 (± 0.9) | 0.005a |
TIMP-1 (ng/mL) | 7.1 (± 1.4) | 12.4 (± 2.3) | < 0.001a |
PAI-1 (ng/mL) | 0.11 (± 0.05) | 0.17 (± 0.06) | 0.037a |
Table 2 Correlation of steatohepatitis, cardiovascular risk, and microbiota composition
Variable1 | Severity and progression of liver injury | CVR factors and metabolism of lipids | Cardiomyocyte morphometry | Microbiota composition | |||||||||||
Quantification of collagen (picrosirius) | TIMP-1 | MCP-1 | IL-1β | miR-33a | miR-126 | PAI-1 | CRI-I | CRI-II | AC | % Normal CAR | Average area of CAR | % Atrophic CAR | |||
Severity and progression of liver injury | NAFLD score | 0.8792 | 0.7912 | 0.6732 | 0.347 | 0.6392 | -0.7772 | 0.4443 | 0.8092 | 0.8202 | 0.8092 | -0.5193 | -0.6302 | 0.7212 | 0.6942 |
Quantification of collagen (picrosirius) | 0.6112 | 0.4563 | 0.7522 | 0.5713 | -0.6832 | 0.415 | 0.8192 | 0.8212 | 0.8192 | -0.205 | -0.312 | 0.238 | 0.3782 | ||
TIMP-1 | 0.8032 | 0.7262 | 0.7282 | -0.8122 | 0.5353 | 0.6912 | 0.7472 | 0.6912 | -0.6942 | -0.405 | 0.6072 | 0.5392 | |||
MCP-1 | 0.5673 | 0.4923 | -0.6232 | 0.336 | 0.5493 | 0.5613 | 0.5493 | -0.4903 | -0.390 | 0.4983 | 0.2323 | ||||
IL-1β | 0.8092 | -0.6883 | 0.5443 | 0.6453 | 0.6882 | 0.6453 | -0.4373 | -0.393 | 0.382 | 0.2933 | |||||
CVR factors and metabolism of lipids | miR-33a | -0.6552 | 0.363 | 0.5293 | 0.6033 | 0.5293 | -0.7042 | 0.038 | 0.232 | 0.1603 | |||||
miR-126 | -0.6342 | -0.7122 | -0.7302 | -0.7122 | 0.4593 | 0.320 | -0.364 | 0.3682 | |||||||
PAI-1 | 0.4873 | 0.6712 | 0.4873 | -0.317 | 0.389 | -0.289 | 0.103 | ||||||||
CRI-I | 0.8632 | 1.0002 | -0.234 | -0.4593 | 0.386 | 0.4692 | |||||||||
CRI-II | 0.8632 | -0.399 | -0.4923 | 0.5513 | 0.5842 | ||||||||||
AC | -0.236 | -0.4573 | 0.389 | 0.4772 | |||||||||||
Cardiomyocyte morphometry | % Normal cardiomyocytes | 0.105 | -0.058 | ||||||||||||
% Average area of cardiomyocytes | -0.8182 | ||||||||||||||
% Atrophic cardiomyocytes |
Table 3 Correlation of gut microbiota at family level, steatohepatitis, and cardiovascular risk factors
Variable1 (Family) | Severity and progression of liver injury | CVR factors and metabolism of lipids | ||||||||
NAFLD score | Quantification of collagen (picrosirius) | TIMP-1 | MCP-1 | miR-33a | miR-126 | PAI-1 | CRI-I | CRI-II | AC | |
Actinomycetaceae | 0.5842 | |||||||||
Aerococcaceae | ||||||||||
Anaeroplasmataceae | -0.5532 | -0.6142 | -0.6142 | |||||||
Atopobiaceae | 0.6272 | 0.6102 | 0.5922 | 0.6632 | 0.5922 | |||||
Bacillales_unclassified | 0.5492 | -0.5482 | -0.5332 | -0.5482 | ||||||
Bacteroidaceae | 0.8362 | 0.7462 | 0.7842 | 0.6892 | -0.7542 | 0.6622 | 0.7322 | 0.6622 | ||
Bacteroidales_unclassified | -0.5602 | -0.5892 | -0.4922 | |||||||
Burkholderiaceae | 0.5642 | |||||||||
Clostridiaceae | 0.8072 | 0.7232 | 0.6452 | 0.5932 | -0.6692 | 0.6762 | 0.6382 | 0.6762 | ||
Clostridiales_unclassified | -0.6282 | -0.5292 | -0.5352 | -0.5762 | -0.5862 | -0.5252 | ||||
Clostridiales_vadinBB60 | -0.6022 | -0.6712 | -0.5272 | -0.5582 | 0.5242 | -0.6262 | -0.5022 | -0.6262 | ||
Corynebacteriaceae | -0.6692 | -0.5452 | -0.6802 | -0.7822 | 0.6112 | -0.5712 | -0.6222 | -0.5712 | ||
Desulfovibrionaceae | -0.8062 | -0.6032 | -0.8722 | -0.7762 | -0.6312 | 0.7552 | -0.7292 | -0.7462 | -0.7292 | |
Eggerthellaceae | 0.4902 | |||||||||
Firmicutes_unclassified | -0.7972 | -0.6372 | -0.6872 | -0.6552 | 0.5942 | -0.6292 | -0.6992 | -0.6292 | ||
Gastranaerophilales | -0.8222 | -0.6562 | -0.6442 | -0.6432 | 0.6572 | -0.6982 | -0.5862 | -0.6982 | ||
Lachnospiraceae | -0.8502 | -0.6532 | -0.7892 | -0.7882 | -0.6132 | 0.7662 | -0.6432 | -0.6292 | -0.6432 | |
Lactobacillaceae | -0.6162 | -0.6332 | 0.7952 | -0.5292 | ||||||
Lactobacillales_unclassified | ||||||||||
Micrococcaceae | 0.6692 | 0.5342 | -0.5282 | 0.4932 | ||||||
Mollicutes_RF39_fa | -0.6502 | -0.6182 | -0.5902 | -0.6092 | 0.7132 | -0.8572 | -0.7682 | -0.8572 | ||
Moraxellaceae | -0.6692 | -0.5362 | -0.5572 | -0.5432 | -0.5992 | -0.4732 | -0.5992 | |||
Muribaculaceae | -0.8162 | -0.7942 | -0.5762 | 0.6932 | -0.6842 | -0.8272 | -0.8462 | -0.8272 | ||
Pasteurellaceae | ||||||||||
Prevotellaceae | -0.7052 | 0.6032 | -0.5222 | -0.4862 | ||||||
Rikenellaceae | -0.6792 | |||||||||
Saccharimonadaceae | -0.7372 | -0.5592 | -0.6192 | -0.6742 | 0.6562 | -0.7762 | -0.7592 | -0.7762 | ||
Staphylococcaceae | -0.7342 | -0.6472 | -0.8082 | -0.8382 | 0.7162 | -0.6162 | -0.6792 | -0.6162 | ||
Streptococcaceae | 0.7902 | 0.7262 | 0.6372 | 0.5952 | -0.6222 | 0.7242 | 0.5152 |
- Citation: Longo L, Rampelotto PH, Filippi-Chiela E, de Souza VEG, Salvati F, Cerski CT, da Silveira TR, Oliveira CP, Uribe-Cruz C, Álvares-da-Silva MR. Gut dysbiosis and systemic inflammation promote cardiomyocyte abnormalities in an experimental model of steatohepatitis. World J Hepatol 2021; 13(12): 2052-2070
- URL: https://www.wjgnet.com/1948-5182/full/v13/i12/2052.htm
- DOI: https://dx.doi.org/10.4254/wjh.v13.i12.2052