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©The Author(s) 2020.
World J Hepatol. Oct 27, 2020; 12(10): 792-806
Published online Oct 27, 2020. doi: 10.4254/wjh.v12.i10.792
Published online Oct 27, 2020. doi: 10.4254/wjh.v12.i10.792
Table 1 Clinical and demographic characteristics of nonalcoholic fatty liver disease patients and controls
Variables | NAFLD, n = 148 | Controls, n = 137 | P value |
Female | 111 (75.0%) | 97 (70.8%) | 0.426 |
Age (yr) | 57 (46.3-63) | 55 (43 61.5) | 0.610 |
BMI (kg/m2) | 32.8 ± 4.8 | 25.4 ± 3.3 | < 0.0001 |
Hypertension | 98 (66.2%) | 24 (17.5%) | < 0.0001 |
Diabetes mellitus | 69 (46.6%) | 12 (8.8%) | < 0.0001 |
Dyslipidemia | 108 (73.0%) | 19 (13.5%) | < 0.0001 |
Fasting glucose (mg/dL) | 101 (90-124.8) | 88 (83-98) | < 0.0001 |
AST (U/L) | 34 (25.5-56.5) | 24 (20.3-29) | < 0.0001 |
ALT (U/L) | 38 (28-70.5) | 29.5 (23-38) | < 0.0001 |
GGT (U/L) | 53 (29-116) | 28 (18.5-55.5) | < 0.0001 |
ALP (U/L) | 97 (74-116.8) | 75 (58.3-83.8) | < 0.0001 |
Total bilirubin (mg/dL) | 0.6 (0.5-0.9) | 0.7 (0.5-0.8) | 0.438 |
Triglycerides (mg/dL) | 162.5 (110-205.3) | 126.5 (93-176) | 0.006 |
Total cholesterol (mg/dL) | 195.8 ± 41.5 | 179.9 ± 47.1 | 0.006 |
VLDL (mg/dL) | 32 (22-42.3) | 24.5 (17.3-33.8) | 0.001 |
HDL (mg/dL) | 48 (39.5-54) | 47 (40.5-60.8) | 0.404 |
LDL (mg/dL) | 107.5 (89.5-137.9) | 96 (67.8-117.5) | 0.009 |
Platelets (109/L) | 219.4 ± 81.8 | 254.6 ± 82.4 | 0.439 |
Ferritin (ng/mL) | 89 (42.7-168.5) | 32 (32-32) | 0.349 |
D vitamin (ng/mL) | 22 (19-27.7) | 26.95 (20.5-32.5) | 0.139 |
Table 2 Clinical and demographic characteristics of the nonalcoholic steatohepatitis and simple steatosis population
Variables | NASH, n = 54 | Simple steatosis, n = 94 | P value |
Female | 44 (81.5%) | 67 (71.3%) | 0.169 |
Age (yr) | 59 (45.8-63) | 57 (47.8-64) | 0.935 |
BMI (kg/m2) | 32.7 ± 4.8 | 32.8 ± 4.8 | 0.968 |
WC | 106.6 (100-114) | 105.3 (96-113.8) | 0.306 |
Arterial hypertension | 39 (72.2%) | 59 (62.8%) | 0.886 |
Diabetes mellitus | 35 (64.8%) | 34 (36.2%) | 0.007 |
Dyslipidemia | 45 (83.3%) | 63 (67.0%) | 0.248 |
Fasting insulin | 16.5 (11.9-31) | 14 (8-18) | 0.055 |
Fasting glucose (mg/dL) | 112.5 (91-151.5) | 98 (90-112) | 0.019 |
AST (U/L) | 53.5 (31.7-69) | 30 (23-38) | < 0.001 |
ALT (U/L) | 61 (36-79.3) | 33 (24-49) | < 0.001 |
GGT (U/L) | 87 (51.7-164.3) | 39 (27-73.3) | < 0.001 |
ALP (U/L) | 99 (78-127.5) | 88 (72-116) | 0.075 |
Total bilirubin (mg/dL) | 0.59 (0.5-0.9) | 0.6 (0.5-0.9) | 0.986 |
Triglycerides (mg/dL) | 162 (113-227.8) | 163 (109.25-201) | 0.544 |
Total cholesterol (mg/dL) | 196.4 ± 39.9 | 195.4 ± 42.7 | 0.793 |
VLDL (mg/dL) | 32 (22.8-45.3) | 32 (22-40.8) | 0.440 |
HDL (mg/dL) | 46 (37.8-53) | 48 (40-55) | 0.432 |
LDL (mg/dL) | 108.5 (89-138.4) | 107 (88.5-137.4) | 0.793 |
Platelets (109/L) | 184 ± 67.4 | 308 ± 1.4 | 0.053 |
Ferritin (ng/mL) | 109 (56.75-267.5) | 69 (37.2-143.8) | 0.019 |
D vitamin (ng/mL) | 21.1 (17.55-25.9) | 23.4 (19-28) | 0.086 |
HOMA-IR | 5.26 (2.97-8.8) | 3.36 (1.77-4.7) | 0.330 |
Table 3 PNPLA3 rs738409 genotype frequencies in nonalcoholic fatty liver disease patients and controls
Genotype frequency % (n) | P value | |||
CC | CG | GG | ||
NAFLD | 37.2 (55) | 43.9 (65) | 18.9 (28) | < 0.001 |
Controls | 58.4 (80) | 31.4 (43) | 10.2 (14) |
Table 4 Association between the PNPLA3 rs738409 genotype and nonalcoholic fatty liver disease or nonalcoholic steatohepatitis, adjusted for age, gender, body mass index and diabetes
Genotypes | OR | 95%CI | P value |
NAFLD vs Control | |||
CC vs CG/GG | 1.69 | 1.21–2.36 | 0.002 |
CC vs GG | 3.13 | 1.49–6.57 | 0.003 |
NASH vs SS | |||
CC vs CG/GG | 3.50 | 1.84–6.64 | < 0.001 |
CC vs GG | 5.53 | 2.04-14.92 | 0.001 |
Table 5 Association of the PNPLA3 rs738409 genotype and activity of nonalcoholic steatohepatitis and fibrosis, adjusted for age, gender, body mass index and diabetes, evaluated by multivariate binary logistic regression analysis
Genotypes | OR | 95%CI | P value |
Activity of NASH (A > 2 vs A < 2) | |||
CC vs CG/GG | 0.65 | 0.22-1.88 | 0.433 |
CC vs GG | 17.11 | 1.87 - 156.25 | 0.012 |
Fibrosis (presence vs absence) | |||
CC vs CG/GG | 3.05 | 1.01-9.17 | 0.046 |
CC vs GG | 7.42 | 1.55-35.47 | 0.012 |
Table 6 TM6SF2 rs58542926 genotype frequencies in nonalcoholic fatty liver disease patients and controls
Genotype frequency % (n) | P value | |||
CC | CT | TT | ||
NAFLD | 83.2 (125) | 14.9 (22) | 0.7 (1) | 0.78 |
Controls | 84.5 (114) | 16.1 (22) | 0.7 (1) |
- Citation: Lisboa QC, Nardelli MJ, Pereira PA, Miranda DM, Ribeiro SN, Costa RSN, Versiani CA, Vidigal PVT, Ferrari TCA, Couto CA. PNPLA3 and TM6SF2 polymorphisms in Brazilian patients with nonalcoholic fatty liver disease. World J Hepatol 2020; 12(10): 792-806
- URL: https://www.wjgnet.com/1948-5182/full/v12/i10/792.htm
- DOI: https://dx.doi.org/10.4254/wjh.v12.i10.792