Retrospective Study
Copyright ©The Author(s) 2017.
World J Hepatol. Sep 28, 2017; 9(27): 1115-1124
Published online Sep 28, 2017. doi: 10.4254/wjh.v9.i27.1115
Figure 1
Figure 1 Polyclonal response of T cells upon recall with peptides of the glutathione S-transferase T1 protein. Percentages of activation upon exposure to the different pools are represented. Three cell types showed activation at different levels. A: Only CD8+ lymphocytes of patients included in group 1 showed activation. The numbers in the figure represent the highest value obtained for each pathway when activation was induced by different pools of peptides; B: Activation of CD4+ lymphocytes was observed in patients of groups 1 and 3; C: CD4+CD8low cells showed the highest percentage of activation. 1Indicate that the analysis could not be performed in these patients.
Figure 2
Figure 2 Different areas of the glutathione S-transferase T1 protein induce different degrees of activation in patients’ cells. A: The majority of CD4+ T cells that showed some degree of activation recognized peptides in pools 5 (blue) and 3 (green) but never in pool 4 (purple); B: CD4+CD8low cells became stimulated almost homogeneously with in pools 3 (green) and 4 (purple); C: CD8+ lymphocytes that showed activation recognized peptides in different pools but never in pool 4 (purple).
Figure 3
Figure 3 Representative plots showing CD4+CD8low T lymphocyte activation in terms of cytokine production. Production of IL-4 and/or IFNγ was analyzed after exposure of the cells to the glutathione S-transferase T1 peptides. As an example, patient 3 had 78.85% of the double positive cells activated with pool 3 and 2.9% with pool 6. Patient 4 had 6.05% of cells activated with pool 3, whereas pool 5 did not have any effect. Patient 17 showed 21.21% of cell activation with pool 3, but no response was observed when the cells were cultured with pool 2. In all the cases, the cells produced both cytokines, indicating a Th0 type of response.