Melatonin attenuates high fat diet-induced fatty liver disease in rats

Figure 1 Histopathological features of liver samples. A: Normal liver architecture in rats on regular diet plus melatonin; B: Severe fatty liver disease in rats on high fat diet without melatonin; C-F: Attenuated steatosis in rats on high fat diet and melatonin. (Hematoxylin-eosin staining was used, ×100). RD: Regular diet; HFD: High fat diet; ML: Melatonin.

Figure 2 Lipidemic profiles of rats on regular diet or high fat diet, with or without melatonin. A: Serum total cholesterol; B: High-density lipoproteins; C: Low-density lipoproteins; D: Triglycerides. HDL: High-density lipoprotein; LDL: Low-density lipoprotein; TG: Triglycerides; HFD: High fat diet; RD: Regular diet; ML: Melatonin; ●: Indicates outliers.

Figure 3 Hepatic inflammation and oxidative stress assessment. A: Hepatic inflammation represented by serum alanine aminotransferase; B: Aspartate aminotransferase; C: Oxidative stress represented by tissue levels of malondialdehyde; D: Glutathione, in rats on regular or high fat diet, with or without melatonin. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; MDA: Malondialdehyde; GSH: Glutathione; HFD: High fat diet; RD: Regular diet; ML: Melatonin; ●: Indicates outliers.