Basic Study
Copyright ©The Author(s) 2020.
World J Hepatol. Jul 27, 2020; 12(7): 363-377
Published online Jul 27, 2020. doi: 10.4254/wjh.v12.i7.363
Figure 1
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Figure 1 Mean body weight changes in rats in each group. Data at each time point are expressed as mean ± SD. NASH: Non-alcoholic steatohepatitis.
Figure 2
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Figure 2 Representative images on light microscopy using hematoxylin and eosin stain, terminal deoxynucleotidyl transferase dUTP nick end labeling stain for apoptosis, or immunohistochemistry stains for NF-kβ, cytochrome-C and caspase-3. A: Hematoxylin and eosin stain; B: Terminal deoxynucleotidyl transferase dUTP nick end labeling stain; C: Immunohistochemistry stains; D: Cytochrome-C; E: Caspase-3. Control groups are presented on the left column; non-alcoholic steatohepatitis group in the middle and aloe vera group on the right column. Arrowheads point to positive cells. NASH: Non-alcoholic steatohepatitis.
Figure 3
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Figure 3 Hepatic malondialdehyde and glutathione levels in each group. A: Malondialdehyde; B: Glutathione. Data are expressed as mean ± SD. NASH: Non-alcoholic steatohepatitis; MDA: Malondialdehyde; GSH: Glutathione.
Figure 4
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Figure 4 The expression of IL-18, PPAR-γ, and NF-kβ using immunohistochemistry methods. A: IL-18; B: PPAR-γ; C: NF-kβ. The positive cells were counted by Aperio ImageScope software. Data are expressed as mean ± SD. NASH: Non-alcoholic steatohepatitis.
Figure 5
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Figure 5 Representative images on light microscopy using immunohistochemistry stains for IL-18 and PPAR-γ. A: IL-18; B: PPAR-γ. Control groups are presented on the left column; non-alcoholic steatohepatitis group in the middle and aloe vera group on the right column. Arrowheads point to positive cells. NASH: Non-alcoholic steatohepatitis.
Figure 6
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Figure 6 The expression of caspase-3 and cytochrome-C using immunohistochemistry methods, and the degree of hepatocyte apoptosis using terminal deoxynucleotidyl transferase dUTP nick end labeling methods. A: Caspase-3; B: Cytochrome-C; C: Degree of hepatocyte apoptosis. The positive cells were counted by Aperio ImageScope software. Data are expressed as mean ± SD. NASH: Non-alcoholic steatohepatitis; TUNEL: Terminal deoxynucleotidyl transferase dUTP nick end labeling.