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©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Apr 26, 2022; 14(4): 287-302
Published online Apr 26, 2022. doi: 10.4252/wjsc.v14.i4.287
Published online Apr 26, 2022. doi: 10.4252/wjsc.v14.i4.287
Dental stem cell-conditioned medium for tissue regeneration: Optimization of production and storage
Batoul Chouaib, Frédéric Cuisinier, Pierre-Yves Collart-Dutilleul, Laboratory Bioengineering and Nanosciences UR_UM104, University of Montpellier, Montpellier 34000, France
Author contributions: Chouaib B conducted the bibliographic research and selected the targetted articles; Chouaib B and Collart-Dutilleul PY analyzed the selected articles; Chouaib B and Collart-Dutilleul PY wrote the main draft; Cuisinier F corrected the manuscript and supervised the findings of this work; all authors discussed the results and contributed to the final manuscript.
Conflict-of-interest statement: All authors declare that they have no conflict of interest.
PRISMA 2009 Checklist statement: The authors have read the PRISMA 2009 Checklist, and the manuscript was prepared and revised according to the PRISMA 2009 Checklist.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Pierre-Yves Collart-Dutilleul, DDS, PhD, Associate Professor, Laboratory Bioengineering and Nanosciences UR_UM104, University of Montpellier, 545 Avenue du Professeur Jean-Louis Viala, Montpellier 34000, France. pierre-yves.collart-dutilleul@umontpellier.fr
Received: March 28, 2021
Peer-review started: March 28, 2021
First decision: May 12, 2021
Revised: May 19, 2021
Accepted: April 21, 2022
Article in press: April 21, 2022
Published online: April 26, 2022
Peer-review started: March 28, 2021
First decision: May 12, 2021
Revised: May 19, 2021
Accepted: April 21, 2022
Article in press: April 21, 2022
Published online: April 26, 2022
Core Tip
Core Tip: Dental Mesenchymal stem cells (DMSC) effects on tissue regeneration are highly mediated by their secreted substances [conditioned medium (CM)] such as soluble factors and extracellular vesicles. The manufacturing of CM products suffers from variable procedures and protocols leading to different results between studies. Focusing on CM produced from DMSC (DMSC-CM), we reviewed the current parameters and manufacturing protocols, aiming to facilitate the standardization and optimization of manufacturing procedures, in accordance with PRISMA guideline. No clear consensus could be recovered as evidence-based methods, but it clearly appeared that the preconditioning environment had a significant impact on DMSC-CM content and efficiency.