有机食物与麦粒灸对大鼠肠道菌群多样性的影响

表1 引物序列

引物	序列
338F	5'-CCTACGGGAGGCAGCAG-3'
518R	5'-ATTACCGCGGCTGCTGG-3'
GC338F	5'-CGCCCGGGGCGCGCCCCGGGGCGGGGCGGGGGCGCGGGGGGCCTACGGGAGGCAGCAG-3'

表2 细菌多样性分析

分组	均匀度(E)	Shannon指数(H')	丰富度(S)
有机组	0.98±0.00	3.62±0.03	40. 0 0±1.15
常规组	0.98±0.01	3.59±0.12	39.50±3.87
常规+麦粒灸组	0.98±0.00	3.78±0.04	47.25±2.06

表3 代表性条带菌群鉴定结果

Band	最相似菌株	相似百分比	相似菌株登录号	菌门
Band1	Clostridium populeti	100	NR_026103	Firmicutes
Band2	Bacteroides stercoris	95	NR_027196	Bacteroidetes
Band3	Barnesiella intestinihominis	96	NR_041668	Bacteroidetes
Band4	Bacteroides stercoris	99	NR_027196	Bacteroidetes
Band5	Coprococcus comes	99	NR_044048	Firmicutes
Band6	Novosphingobiumnitrogenifigens	99	NR_043857	Proteobacteria
Band7	Flavonifractor plautii	97	NR_043142	Firmicutes
Band8	Clostridium methylpentosum	95	NR_029355	Firmicutes
Band9	Barnesiella intestinihominis	90	NR_041668	Bacteroidetes