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©The Author(s) 2014.
世界华人消化杂志. 2014-01-28; 22(3): 415-422
在线出版 2014-01-28. doi: 10.11569/wcjd.v22.i3.415
在线出版 2014-01-28. doi: 10.11569/wcjd.v22.i3.415
表1 25种血浆miRNA实时荧光定量PCR检测结果
No. | miRNA name | △△Ct | 2-△△Ct | Regulation |
1 | hsa-miR-4284 | -1.9 | 3.66 | Up |
2 | hsa-miR-3620 | -1.6 | 3.09 | Up |
3 | hsa-miR-652-5p | -1.3 | 2.50 | Up |
4 | hsa-miR-4800-5p | -1.1 | 2.08 | Up |
5 | hsa-miR-1249 | -0.9 | 1.92 | N |
6 | hsa-miR-3937 | -0.9 | 1.85 | N |
7 | hsa-miR-584-5p | -0.8 | 1.77 | N |
8 | hsa-miR-1226-5p | -0.8 | 1.75 | N |
9 | hsa-miR-4298 | -0.8 | 1.69 | N |
10 | hsa-miR-4655-3p | -0.6 | 1.57 | N |
11 | hsa-miR-1260a | -0.6 | 1.50 | N |
12 | hsa-miR-1224-5p | -0.6 | 1.48 | N |
13 | hsa-miR-577 | -0.4 | 1.29 | N |
14 | hsa-miR-378i | -0.2 | 1.14 | N |
15 | hsa-miR-663a | 0.0 | 1.03 | N |
16 | hsa-miR-4286 | 0.1 | 0.92 | N |
17 | hsa-miR-7-5p | 0.4 | 0.75 | N |
18 | hsa-miR-34a-5p | 0.6 | 0.66 | N |
19 | hsa-miR-454-3p | 0.6 | 0.65 | N |
20 | hsa-miR-125b-5p | 0.7 | 0.63 | N |
21 | hsa-miR-32-5p | 0.7 | 0.62 | N |
22 | hsa-miR-144-5p | 0.7 | 0.61 | N |
23 | hsa-miR-338-3p | 1.1 | 0.48 | Down |
24 | hsa-miR-424-5p | 1.1 | 0.47 | Down |
25 | hsa-miR-194-5p | 1.3 | 0.39 | Down |
表2 部分miRNA靶基因及其GO生物学功能
序号 | miRNA | 积分 | 靶基因 | 基因全称 | GO生物学功能 |
1 | hsa-miR-4800-5p | 100 | ITGB1BP1 | integrin beta 1 binding protein 1 | 整合素结合蛋白, 细胞黏附功能 |
2 | hsa-miR-4284 | 99 | GNG13 | guanine nucleotide binding protein (G protein), gamma 13 | 水解酶; G蛋白耦联受体蛋白 |
3 | hsa-miR-4284 | 98 | VPS53 | vacuolar protein sorting 53 homolog (S. cerevisiae) | 线粒体内膜蛋白, 参与脂质代谢过程 |
4 | hsa-miR-4284 | 98 | MAVS | mitochondrial antiviral signaling protein | 线粒体信号蛋白 |
5 | hsa-miR-4284 | 97 | ORAI2 | ORAI calcium release-activated calcium modulator 2 | 钙释放激活钙调素 |
6 | hsa-miR-652-5p | 94 | SEC14L1 | SEC14-like 1 (S. cerevisiae) | 胆固醇代谢转运/携带蛋白 |
7 | hsa-miR-4284 | 93 | SPN | sialophorin | 炎细胞表面糖蛋白, 负调控免疫应答 |
8 | hsa-miR-4284 | 91 | LOC100653346 | uncharacterized LOC100653346 | 功能不清 |
9 | hsa-miR-4284 | 91 | TMEM120B | transmembrane protein 120B | 物质代谢过程 |
10 | hsa-miR-4284 | 90 | CYP20A1 | cytochrome P450, family 20, subfamily A, polypeptide 1 | 氧化酶 |
11 | hsa-miR-4284 | 89 | OPA3 | optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia) | 线粒体外膜蛋白 |
12 | hsa-miR-4284 | 89 | CDK17 | cyclin-dependent kinase 17 | 非受体丝/苏氨酸蛋白激酶 |
13 | hsa-miR-4284 | 89 | RAB3B | RAB3B, member RAS oncogene family | 小分子GTP结合蛋白, RAB家族成员 |
14 | hsa-miR-652-5p | 88 | SLC25A24 | solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24 | 线粒体携带因子 |
15 | hsa-miR-4284 | 88 | PLA2R1 | phospholipase A2 receptor 1, 180 kDa | 磷酯酶A2受体 |
16 | hsa-miR-3620 | 88 | USP46 | ubiquitin specific peptidase 46 | 丝氨酸蛋白酶; 泛素-蛋白质连接酶 |
17 | hsa-miR-3620 | 87 | PUM1 | pumilio homolog 1 (Drosophila) | 翻译因子 |
18 | hsa-miR-4800-5p | 87 | DTL | denticleless homolog (Drosophila) | 核基质相关蛋白 |
19 | hsa-miR-4284 | 86 | ZNF292 | zinc finger protein 292 | 核酸酶; 转录因子 |
20 | hsa-miR-4284 | 86 | PLIN5 | perilipin 5 | 脂质转运/携带蛋白 |
21 | hsa-miR-4284 | 86 | ENAH | Enabled homolog (Drosophila) | 细胞骨架结构成份 |
22 | hsa-miR-4284 | 85 | RAB3IP | RAB3A interacting protein (rabin3) | 鸟苷酸交换因子; 信号转导 |
23 | hsa-miR-3620 | 85 | ACPP | acid phosphatase, prostate | 磷酯酶 |
24 | hsa-miR-652-5p | 83 | TLR7 | toll-like receptor 7 | Toll受体信号通路 |
25 | hsa-miR-652-5p | 83 | RRAS2 | related RAS viral (r-ras) oncogene homolog 2 | 小GTP酶 |
26 | hsa-miR-3620 | 83 | GPBP1L1 | GC-rich promoter binding protein 1-like 1 | 功能不清 |
27 | hsa-miR-4284 | 82 | RLIM | ring finger protein, LIM domain interacting | E3泛素蛋白连接酶, 可调控细胞迁移 |
28 | hsa-miR-3620 | 82 | INSIG2 | insulin induced gene 2 | 胆固醇代谢过程 |
29 | hsa-miR-4284 | 82 | ZFR2 | zinc finger RNA binding protein 2 | RNA结合蛋白 |
30 | hsa-miR-652-5p | 81 | UTS2D | urotensin 2 domain containing | 功能不清 |
31 | hsa-miR-652-5p | 81 | PRKCA | protein kinase C, alpha | 非受体丝/苏氨酸蛋白激酶 |
32 | hsa-miR-3620 | 81 | TSPAN9 | tetraspanin 9 | 膜结合信号分子; 受体; 细胞黏附分子 |
33 | hsa-miR-4284 | 80 | AAK1 | AP2 associated kinase 1 | 非受体丝/苏氨酸蛋白激酶 |
引文著录: 叶长根, 孙水林, 白茹, 朱伟星, 陈彩萍, 谢平, 赵晖, 涂文娟, 高得勇, 刘亮明. 抗结核药物肝损伤与无肝损伤患者化疗前血循环miRNA分子的差异性表达. 世界华人消化杂志 2014; 22(3): 415-422