miR495、miR551a靶向干扰PRL-3表达抑制胃癌腹膜转移

表1 miRNA茎环逆转录引物设计

引物名称	引物序列
	RT: 5'- GCGCGTGAGCAGGCTGGAGAAATTAACCACGCGCAAGAAG-3'
miR495	F: 5'-AAACAAACATGGTGCA-3'
	R: 5'-GAGCAGGCTGGAGAA-3'
	RT: 5'-GCGCGTGAGCAGGCTGGAGAAATTAACCACGCGCTGGAAAC-3'
miR551a	F: 5'-GCGACCCACTCTTG-3'
	R: 5'-GAGCAGGCTGGAGAA-3'
	RT: 5'-CGCTTCACGAATTTGCGTGTCAT-3'
U6	F: 5'- CTCGCTTCGGCAGCACATA-3'
	R: 5'- CGCTTCACGAATTTGCGTG-3'

RT: 茎环引物; F: 上游引物; R: 下游引物.

表2 PRL-3的引物设计

引物名称	引物序列
PRL-3	F: 5'- GGGACTTCTCAGGTCGTGTC-3'
	R: 5'- AGCCCCGTACTTCTTCAGGT-3'
β-actin	F: 5'- TCATCACCATTGGCAATGAG-3'
	R: 5'- CACTGTGTTGGCGTACAGGT-3'

PRL-3: 促肝细胞再生磷酸酶-3; F: 上游引物; R: 下游引物.