胰腺癌干细胞差异microRNAs的表达及生物信息学

表1 部分差异miRNAs SYBR Green 荧光定量PCR引物设计

miRNAs	引物
U6	ttcgtgaagcgttccatatttt
hsa-miR-125b	tccctgagaccctaacttgtga
hsa-miR-126	tcgtaccgtgagtaataatgcg
hsa-miR-1290	tggatttttggatcaggga
hsa-miR-140-3p	taccacagggtagaaccacgg
hsa-miR-140-5p	cagtggttttaccctatggtag
hsa-miR-148a	tcagtgcactacagaactttgt
hsa-miR-181a	acattcaacgctgtcggtgagt
hsa-miR-181b	aacattcattgctgtcggtgg
hsa-miR-195	tagcagcacagaaatattggc
hsa-miR-23b	atcacattgccagggattacc
hsa-miR-27b	ttcacagtggctaagttctgc
hsa-miR-30a*	ctttcagtcggatgtttgcag
hsa-miR-362-5p	aatccttggaacctaggtgtgagt
hsa-miR-455-3p	gcagtccatgggcatatacac
hsa-miR-532-5p	catgccttgagtgtaggacc
hsa-miR-584	ttatggtttgcctgggactgag
hsa-miR-625	gcagggggaaagttctatagtcc
hsa-miR-660	tacccattgcatatcggagttg
hsa-miR-744	tgcggggctagggctaac
hsa-miR-10a	taccctgtagatccgaatttgtg
hsa-miR-10b	taccctgtagaaccgaatttgtg

表2 荧光定量PCR验证21条差异microRNAs

Sample Name	Average Ct		ΔCt			2 -ΔCt*
	BXPC3	Miapaca2	BXPC3	Miapaca2		BXPC3	Miapaca2
U6	24.54	23.59
hsa-miR-126	26.85	24.38	2.31		0.78	0.20	0.58
hsa-miR-140-3p	27.76	25.68	3.22		2.09	0.11	0.24
hsa-miR-140-5p	31.90	29.81	7.36		6.22	0.006	0.013
hsa-miR-30a*	23.99	22.54	-0.60		-1.06	1.46	2.08
hsa-miR-532-5p	29.01	26.33	4.46		2.74	0.05	0.15
hsa-miR-584	29.33	26.92	4.79		3.33	0.04	0.10
hsa-miR-660	27.25	24.67	2.71		1.07	0.15	0.48
hsa-miR-744	24.34	22.98	-0.20		-0.61	1.15	1.53
hsa-miR362-5p	28.11	24.76	3.57		1.17	0.08	0.45
hsa-mir-10a	25.62	23.67	1.08		0.08	0.47	0.95
hsa-mir-10b	27.03	24.03	2.47		0.43	0.18	0.74
hsa-miR1290	20.61	20.22	-3.93		-3.37	15.24	10.35
hsa-miR455-3p	23.42	27.15	-1.12		3.56	2.17	0.08
hsa-mir-125b	24.00	28.22	-0.55		4.63	1.46	0.04
hsa-miR-148a	25.64	29.18	1.09		5.59	0.47	0.02
hsa-miR-181a	22.08	25.29	-2.46		1.70	5.51	0.31
hsa-miR-181b	21.84	24.72	-2.71		1.13	6.53	0.45
hsa-miR-195	23.47	23.20	-1.07		-0.39	2.10	1.31
hsa-miR-23b	19.05	21.07	-5.50		-2.52	45.20	5.74
hsa-miR-27b	21.64	25.95	-2.90		2.35	7.47	0.20
hsa-miR-625	25.35	27.51	0.80		3.92	0.57	0.07

2 ^-△Ct*表示Miapaca2组目的基因的表达相对于BXPC3组的变化倍数.