大肠腺瘤与大肠腺癌肿瘤相关基因的差异表达

表1 样品总RNA的A₂₆₀、A₂₈₀、A₂₆₀/A₂₈₀比值、RNA浓度、RNA总量

样品	A260	A280	A260/A280	RNA浓度(mg/L)	RNA总量(μg)
大肠腺瘤	1.602	0.796	2.02	6.410	192
大肠腺癌	0.456	0.244	1.97	1.824	18.24

表2 RT-PCR技术与芯片技术检测结果的比较

基因功能	基因标签	Ratio
		芯片技术	RT-PCR技术
Stratifin	SFN	4.22	4.18
jun B proto-oncogene	JUNB	12.25	11.86
immediate early response 3	IER3	16.83	15.19
calpain 1, (mu/I) large subunit	CAPN1	14.75	15.30

表3 仅在大肠腺瘤中差异表达的肿瘤相关基因

GenBank序列号	基因功能	基因标签	Ratio
			大肠腺瘤	大肠腺癌
AA496997	lamin A/C	LMNA	0.24	-
N71003	programmed cell death 4	PDCD4	0.21	-
AA436401	TU3A protein	TU3A	0.45	-
AI817942	zeta-chain associated protein kinase (70 000 Da)	ZAP70	0.30	-
AA447515	Mad4 homolog	MAD4	0.31	-
AA630800	interferon, gamma-inducible protein 30	IFI30	0.47	-
W47350	retinoic acid receptor responder 3	RARRES3	0.18	-
AI650283	serum/glucocorticoid regulated kinase 2	SGK2	0.45	-
AA400973	lipocalin 2 (oncogene 24p3)	LCN2	3.16	-

表4 仅在大肠腺癌中差异表达的肿瘤相关基因(-表示基因表达下调)

GenBank序列号	基因功能	基因标签	Ratio
			大肠腺瘤	大肠腺癌
NM001170341	myeloid leukemia factor 2	MLF2	-	2.05
NM005438	FOS-like antigen-1	FOSL1	-	2.09
BC059522	ribosomal protein S30	FAU	-	2.12
BC08072	v-raf murine sarcoma 3611 viral oncogene homolog 1	ARAF1	-	2.20
NM020531	chromosome 20open reading frame 3	C20ORF3	-	2.23
NM008583	multiple endocrine neoplasia I	MEN1	-	2.23
NM204434	cyclin-dependent kinase inhibitor 2A	CDKN2A	-	2.27
NM133862	fibrinogen, gamma polypeptide	FGG	-	2.75
BC162533	GRO2 oncogene	GRO2	-	2.93
NM011492	serine/threonine kinase 11 (Peutz-Jeghers syndrome)	STK11	-	3.02
NM205510	fibroblast growth factor receptor 1	FGFR1	-	3.52
NM001040403	prohibitin	PHB	-	3.66
NM00109824	v-Ha-ras Harvey rat sarcoma viral oncogene homolog	HRAS	-	4.03
NM001950	E2F transcription factor 4,p107/p130-binding	E2F4	-	4.08
NM001082478	insulin-like growth factor 2 receptor	IGF2R	-	4.17
NM004448	v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2	ERBB2	-	4.57
NM001107159	matrix metalloproteinase 19	MMP19	-	4.60
NM010658	v-maf musculoaponeurotic fibrosarcoma oncogene family	MAFG	-	4.85
NM033158	hyaluronoglucosaminidase 2	HYAL2	-	5.26
NM022012	mitogen-activated protein kinase kinase kinase 11	MAP3K11	-	5.34
NM023983	melanoma adhesion molecule	MCAM	-	5.48
NM001170716	breast cancer anti-estrogen resistance 1	BCAR1	-	6.16
NM000535	postmeiotic segregation increased (S. cerevisiae) 2	PMS2	-	6.18
BC046375	P53-induced protein	PIG11	-	7.66
NM001142573	IMP (inosine monophosphate) dehydrogenase 1	IMPDH1	-	7.67
NM005380	neuroblastoma, suppression of tumorigenicity 1	NBL1	-	7.69
NM003542	H4 histone family, member G	H4FG	-	8.13
NM002466	v-myb avian myeloblastosis viral oncogene homolog-like 2	MYBL2	-	10.76
NM022588	metastasis associated 1	MTA1	-	12.03
NM194359	v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3	ERBB3	-	-6.02
L36953	MAD (mothers against decapentaplegic) homolog 4	MADH4	-	-5.03
NM005638	ADP-ribosylation factor GTPase activating protein 1	ARFGAP	-	-4.76
NM032415	mucosa associated lymphoid tissue lymphoma translocation gene 1	MALT1	-	-3.27
NM008284	v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog	KRAS2	-	-3.26
XM002190948	v-ral simian leukemia viral oncogene homolog B	RALB	-	-3.21
NM003766	coiled-coil, myosin-like BCL2-interacting protein	BECN1	-	-3.15
NM001005765	RAP1A, member of RAS oncogene family	RAP1A	-	-3.08
NM002439	phosphatase and tensin homolog	PTEN	-	-3.05
XM002084629	TRK-fused gene	TFG	-	-3.03
AY805747	ras homolog gene family, member E	ARHE	-	-2.95
NM001099373	SET translocation	SET	-	-2.93
NM053455	fibrinogen-like 2	FGL2	-	-2.70
NM012680	tuberous sclerosis 1	TSC1	-	-2.67
NM001146216	transforming acidic coiled-coil containing protein 1	TACC1	-	-2.59
NM017045	retinoblastoma 1 (including osteosarcoma)	RB1	-	-2.42
NM133250	mutS (E. coli) homolog 2	MSH2	-	-2.24
NM005805	cadherin 1, type 1, E-cadherin (epithelial)	CDH1	-	-2.10

表5 大肠腺瘤与大肠腺癌中均差异表达的肿瘤相关基因(-表示基因 表达下调)

GenBank序列号	基因标签	基因标签	Ratio
			大肠腺瘤	大肠腺癌
H15456	calpain 1, (mu/I) large subunit	CAPN1	0.36	14.75
AI677994	fms-related tyrosine kinase 3 ligand	FLT3LG	0.30	2.09
AA464600	v-myc avian myelocytomatosis viral oncogene homolog	MYC	2.85	3.90
AA460168	growth arrest and DNA-damage-inducible 34	GADD34	0.25	3.70
AA495936	microsomal glutathione S-transferase 1	MGST1	2.18	-2.87
AA191692	stratifin	SFN	0.36	4.22
AA043501	v-maf musculoaponeurotic fibrosarcoma (avian) oncogene homolog	MAF	0.36	4.85
H23235	platelet-derived growth factor receptor	PDGFRA	0.44	-3.24
N94468	jun B proto-oncogene	JUNB	0.33	12.25
AA426216	malignant cell expression-enhanced gene/tumor progression-enhanced gene	LENG4	0.49	5.80
AA486219	SRp25 nuclear protein	LOC51329	0.26	9.77
AA434373	E74-like factor 3 (epithelial-specific)	ELF3	0.33	13.54
AA485377	v-fos FBJ murine osteosarcoma viral oncogene homolog	FOS	0.32	2.09
T61948	FBJ murine osteosarcoma viral oncogene homolog B	FOSB	0.28	11.31
AA463204	pleiomorphic adenoma gene-like 1	PLAGL1	2.54	-2.47
AA430032	pituitary tumor-transforming 1	PTTG1	2.64	15.76
AA457705	immediate early response 3	IER3	0.21	16.83