基础研究
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©The Author(s) 2011. Published by Baishideng Publishing Group Inc. All rights reserved.
世界华人消化杂志. 2011-07-08; 19(19): 2011-2015
在线出版 2011-07-08. doi: 10.11569/wcjd.v19.i19.2011
表1 标准曲线数据
取样量(mL) 容量瓶体积(mL) 芦丁浓度(g/L) 吸光度(A ) 5 100 0.00590 0.208 2 25 0.00944 0.317 5 50 0.01180 0.415 10 50 0.02360 0.832 10 25 0.04720 1.641
表2 不同浓度的水提黄酮类化合物对结肠癌细胞株增殖的抑制率 (mean±SD, %)
浓度(mg/L) 24 h 72 h LoVo SW480 HT-29 HCT-8 LoVo SW480 HT-29 HCT-8 2.5 3.15±2.23 4.48±10.95 7.90±10.72 3.79±4.63 2.50±2.25 11.82±10.63 13.69±5.46 1.40±7.15 5.0 6.78±2.80 8.36±11.77a 14.45±5.14 10.13±3.31a 9.49±3.87 33.27±8.42 14.36±3.05 19.59±4.50 10.0 10.84±3.35 13.67±6.60a 19.27±3.76 22.36±1.40 9.85±1.36 37.77±5.84 17.04±2.04 23.25±3.58 20.0 13.34±2.67 17.53±7.78a 21.65±1.31 34.62±2.32 16.12±1.93 52.35±5.72 21.82±5.90 34.54±5.34 40.0 17.83±1.63a 20.84±7.80a 22.23±2.13 37.51±0.58a 34.51±5.26 68.17±7.76 25.02±15.42 54.51±5.15 60.0 24.34±2.54a 32.79±4.69a 24.20±2.41a 49.75±4.89 56.37±2.31 71.08±8.16 49.15±5.64 57.02±6.76 80.0 30.37±2.05a 41.69±0.89a 32.25±3.70a 52.50±3.39a 66.43±2.76 76.00±5.87 56.25±2.09 69.87±3.52
表3 不同浓度的醇提黄酮类化合物对结肠癌细胞株增殖的抑制率 (mean±SD, %)
浓度(mg/L) 24 h 72 h LoVo SW480 HT-29 HCT-8 LoVo SW480 HT-29 HCT-8 2.5 2.40±0.95 3.90±1.69a 2.67±2.65 2.84±2.31 12.59±7.03 9.41±2.88 4.05±6.91 10.09±5.31 5.0 5.56±1.21a 8.24±2.97 6.78±0.67 10.22±7.49 17.66±2.80 13.28±11.96 6.24±13.51 13.02±6.02 10.0 6.97±0.79a 12.03±4.67 8.44±1.82 21.40±1.27 20.36±3.54 17.36±5.24 8.28±9.01 22.66±8.12 20.0 8.71±1.15a 14.71±3.82 14.90±10.27 27.42±5.99 21.52±4.60 24.15±5.54 10.64±10.88 26.52±7.74 40.0 11.87±0.93a 18.02±4.14a 16.37±5.59 29.68±5.95 25.49±3.47 32.79±6.02 36.57±12.85 38.37±1.70 60.0 17.00±2.06a 21.94±4.81a 24.25±11.29 30.30±4.17a 39.53±2.18 47.55±2.45 42.63±14.22 44.82±5.99 80.0 22.34±2.61a 29.91±3.43a 27.72±13.86 33.59±2.39a 53.90±1.81 60.35±6.61 50.04±8.89 47.37±2.71
表4 洋葱提取物对不同细胞的IC50 (mg/L)
细胞 24 h 72 h 水 乙醇 水 乙醇 LoVo 426.120 1 246.504 55.106 141.085 SW480 193.312 618.633 17.005 71.438 HT-29 94.883 463.346 17.005 84.062 HCT-8 62.612 223.163 35.823 92.338
表5 24 h相同浓度下水提物与醇提物对结肠癌细胞株增殖的抑制作用 (mean±SD, %)
浓度(mg/L) 水提物 醇提物 LoVo SW480 HT-29 HCT-8 LoVo SW480 HT-29 HCT-8 2.5 3.15±2.23 4.48±10.95 7.90±10.72 3.79±4.63 2.40±0.95 3.90±1.69 2.67±2.65 2.84±2.31 5.0 6.78±2.80 8.36±11.77 14.45±5.14 10.13±3.31 5.56±1.21 8.24±2.97 6.78±0.67 10.22±7.49 10.0 10.84±3.35 13.67±6.60 19.27±3.76a 22.36±1.40 6.97±0.79 12.03±4.67 8.44±1.82 21.40±1.27d 20.0 13.34±2.67 17.53±7.78 21.65±1.31 34.62±2.32d 8.71±1.15 14.71±3.82 14.90±10.27 27.42±5.99 40.0 17.83±1.63a 20.84±7.80 22.23±2.13 37.51±0.58d 11.87±0.93 18.02±4.14 16.37±5.59 29.68±5.95d 60.0 24.34±2.54a 32.79±4.69a 24.20±2.41 49.75±4.89a d 17.00±2.06 21.94±4.81 24.25±11.29 30.30±4.17 80.0 30.37±2.05a 41.69±0.89a 32.25±3.70 52.50±3.39a d 22.34±2.61 29.91±3.43 27.72±13.86 33.59±2.39
表6 72 h相同浓度下水提物与醇提物对结肠癌细胞株增殖的抑制作用 (mean±SD, %)
浓度(mg/L) 24 h 72 h LoVo SW480 HT-29 HCT-8 LoVo SW480 HT-29 HCT-8 2.5 2.50±2.25 11.82±10.63 13.69±5.46 1.40±7.15 12.59±7.03 9.41±2.88 4.05±6.91 10.09±5.31 5.0 9.49±3.87a 33.27±8.42 14.36±3.05 19.59±4.50 17.66±2.80 13.28±11.96 6.24±13.51 13.02±6.02 10.0 9.85±1.36a 37.77±5.84a d 17.04±2.04 23.25±3.58 20.36±3.54 17.36±5.24 8.28±9.01 22.66±8.12 20.0 16.12±1.93 52.35±5.72a d 21.82±5.90 34.54±5.34 21.52±4.60 24.15±5.54 10.64±10.88 26.52±7.74 40.0 34.51±5.26 68.17±7.76a 25.02±15.42 54.51±5.15a 25.49±3.47 32.79±6.02 36.57±12.85 38.37±1.70 60.0 56.37±2.31a 71.08±8.16a 49.15±5.64 57.02±6.76 39.53±2.18 47.55±2.45 42.63±14.22 44.82±5.99 80.0 66.43±2.76a 76.00±5.87a 56.25±2.09 69.87±3.52a 53.90±1.81 60.35±6.61 50.04±8.89 47.37±2.71
引文著录: 周阿成, 金黑鹰, 谈瑄忠, 钱晓磊, 张春霞, 何勇山, 王水明. 洋葱提取物对结肠癌细胞增殖的抑制作用. 世界华人消化杂志 2011; 19(19): 2011-2015