Copyright
©The Author(s) 2008.
世界华人消化杂志. 2008-02-08; 16(4): 426-430
在线出版 2008-02-08. doi: 10.11569/wcjd.v16.i4.426
在线出版 2008-02-08. doi: 10.11569/wcjd.v16.i4.426
表1 RT-PCR检测引物序列表
| Gene | Accession No. | Primer sequence | Position | Tm(℃) | Products |
| Dnmt1 | NM_001379 | 5'-TCCACAGTGTTCACAGAGG-3' | 3787-4013 | 54.0 | 227 bp |
| 5'-CGGTAGTAGTCGCAGTAGC-3' | |||||
| Dnmt3a | NM_175629 | 5'-GAAGCGGGCAAAGAACAG-3' | 780-1024 | 52.7 | 245 bp |
| 5'-TCTTCCACAGCATTCAATTCC-3' | |||||
| Dnmt3b | NM_006892 | 5'-GCTCTTACCTTACCATCG-3' | 809-937 | 48.2 | 165 bp |
| 5'-GATACTCTGAACTGTCTCC-3' | |||||
| HMT | NM_024757 | 5'-TCATCAGTCGCTACCTCAGAAC-3' | 749-1023 | 57.3 | 275 bp |
| 5'-AGTCCATCTCCAGGCTCTCC-3' | |||||
| 18S | M10098 | 5'-CGGCTACCACATCCAAGGAA-3' | 551-750 | 60.0 | 200 bp |
| 5'-GCTGGAATTACCGCGGCT-3' |
引文著录: 翟荣林, 蔡开琳, 王国斌, 许飞, 田元, 张景辉. Dnmt1, Dnmt3a, Dnmt3b和HMT在不同结肠癌细胞系的表达及意义. 世界华人消化杂志 2008; 16(4): 426-430