舒林酸对人结肠癌细胞凋亡及相关基因表达谱的作用

表1 实验样品总RNA质量测定

分组	纯化前总量(μg)	A260/280	电泳检测
处理组	1203	2.06	合格
对照组	2131.02	2.12	合格

表2 表达水平上调或下调明显且与凋亡或肿瘤相关的基因

GenBank	基因名称	基因简写	Ratio(Cy3/Cy5)
NM_001946	Dual specificity phosphatase 6	DUSP6	4.084
NM_000389	Cyclin-dependent kinas inhibitor 1A (p21, Cip1)	CDKN1A	4.139
NM_002203	Integrin, alpha 2(CD49B, alpha 2 subunit of VLA-2 receptor)	ITGA2	4.151
AF332558	BCL2 binding component 3	BBC3	4.383
NM_002818	Proteasome (prosome, macropain) activator subunit 2 (PA28 beta)	PSME2	5.277
NM_003330	Thioredoxin reductase 1	TXNRD1	5.36
NM_002229	Jun B proto-oncogene	JUNB	5.649
NM_005980	S100 calcium binding protein P	S100P	6.143
NM_001964	Early growth response 1	EGR1	8.357
NM_016270	Kruppel-like factor 2 (lung)	KLF2	9.428
NM_001657	Amphiregulin (schwannoma-derived growth factor)	AREG	11.54
NM_004864	Growth differentiation factor 15	GDF15	13.88
NM_001168	Baculoviral IAP repeat-containing 5 (survivin)	BIRC5	0.113
NM_012291	Extra spindle poles like 1 (S. cerevisiae)	ESPL1	0.119
NM_007019	Ubiquitin-conjugating enzyme E2C	UBE2C	0.122
NM_001237	Cyclin A2	CCNA2	0.127
NM_002658	Plasminogen activator, urokinase	PLAU	0.144
AF220267	Ubiquitin-like, containing PHD and RING finger domains, 1	UHRF1	0.155
NM_004217	Aurora kinase B	AURKB	0.158
AF070648	Caveolin 1, caveolae protein, 22 kDa	CAV1	0.159
NM_005030	Polo-like kinase 1 (Drosophila)	PLK1	0.167
NM_014750	Discs, large homolog 7 (Drosophila)	DLG7	0.184
NM_016426	G-2 and S-phase expressed 1	GTSE1	0.192