应用基因表达谱芯片技术筛选GABA作用下肝星状细胞差异表达基因

表1 表达显著增强的基因

序号	GenBank登录号	编码蛋白	Cy5/Cy3
1	M27812	突触蛋白Ia(synapsin Ia)	2.018
2	AF121265	β-连锁蛋白(beta-catenin)	2.030
3	J05214	5'-核苷酸酶(5'-nucleotidase)	2.086
4	BC062235	异种核蛋白-a1(heterogeneous nuclear ribonucleoprotein A1)	2.09
5	BC081810	法尼基转移酶1 (farnesyl diphosphate farnesyl transferase 1)	2.120
6	U34843	细胞周期相关蛋白(cell cycle progression related D123)	2.129
7	BC062034	醛类还原酶(Aldehyde reductase 1)	2.209
8	AF476963	结合珠蛋白(haptoglobin-like protein)	2.364
9	L27111	刺激激活的蛋白激酶(stress activated protein kinase alpha I )	2.435
10	BC078869	CTL靶抗原(CTL target antigen )	2.460
11	BC060556	核糖核蛋白体结合蛋白(ribophorin II)	2.725

表2 表达显著下降的基因

序号	GenBank登录号	编码蛋白	Cy5/Cy3
1	L27084	果糖-2, 6-磷酸酶fructose-6-phosphate, 2-kinase: fructose-2, 6-bisphosphatase)	0.206
2	BC088090	视黄醇脱氢酶3(retinol dehydrogenase type III)	0.213
3	BC081844	岩藻糖苷酶a-L-1(fucosidase, alpha-L-1)	0.239
4	BC088847	磷酸果糖激酶(phosphofructokinase)	0.268
5	AY323237	辅酶Q结合蛋白(low molecular mass ubiquinone-binding protein)	0.274
6	AB039663	孔蛋白2(VDAC2)	0.281
7	AY177703	r-酪氨酸磷酸酶(receptor-like protein tyrosine phosphatase gamma A-type isoform)	0.304
8	BC079369	丙酮酸脱氢酶E1 (pyruvate dehydrogenase E1)	0.338
9	BC088166	a1-微球蛋白 (alpha 1microglobulin/bikunin)	0.339
10	U17253	转录抑制因子NAB1(transcriptional repressor NAB1)	0.342
11	AF461496	柠檬酸合酶前体(citrate synthase precursor)	0.368
12	BC061819	甘露糖苷酶2(mannosidase 2)	0.378
13	AB097077	核蛋白UKP83(nuclear protein UKp83)	0.383
14	U76714	细胞黏附调节因子(cell adhesion regulator)	0.385
15	M11185	髓磷脂脂蛋白(myelin proteolipid protein)	0.397
16	J02679	甲萘醌氧化还原酶(menadione oxidoreductase)	0.397
17	BC091363	维甲类受体(retinoid X receptor gamma)	0.400
18	BC078838	肾特异性膜蛋白(kidney-specific membrane protein)	0425
19	AB180485	核受体联合激活蛋白P120(nuclear receptor coactivator p120 )	0.426
20	BC081855	磷脂结合蛋白(annexin A5)	0.430
21	AF121217	前-a-2胶原蛋白(pro-alpha-2(I) collagen)	0.437
22	BC088191	短链脱氢还原酶(short-chain dehydrogenase/reductase)	0.444
23	BC062066	硫辛酰胺脱氢酶(diaphorase)	0.452
24	BC085739	3－羟邻氨苯丙酸－3, 4, －加双氧酶 (3-hydroxyanthranilate 3,4-dioxygenase)	0.472
25	BC088146	C细胞色素P450(cytochrome P450)	0.481
26	M83560	谷氨酸受体5-1(glutamate receptor subunit 5-1)	0.485