用SEREX方法筛选HCC抗原及hcct-19表达谱的检测

表1 SEREX法筛选HCC表达文库所得的基因片段

Clone	Gene	Identity(%)	Accession number	Location	Function
hcct-1, 14	Homo. EIF3S2	99%	U39067	1p34.1	translation initiation factor activity
hcct-3, 6	Homo. RAB4B	98%	NM016154	19q13.2	GTP binding, GTPase activity
hcct-4, 21	Homo. HSPCA	99%	NM005348	14q32.33	Molecule champerone
hcct-5, 8	Homo. IGFBP2	99%	NM000597	2q33-q34	insulin-like growth factor binding
hcct-7	Homo. HSP70	99%	NM004134	5q31.1	Molecule champerone
hcct-9	Homo. MAGE-3	97%	BC005963	Xq28	Unknown
hcct-11, 15	Homo. LDH-A	99%	NM010699	7q	Metabolic enzyme
hcct-12, 16	Homo. MAGE-1	98%	BC017555	Xq28	Unknown
hcct-13	Homo. RFC2	98%	NM181471	7q11.23	nucleotide binding
hcct-18	Homo. hypothetical protein FLJ25976	97%	NM174943	14q24.3	unknown
hcct-19	Homo. MCART1	99%	NM03342	9p13.3-p12	binding
hcct-22	RNA Arabidopsis thaliana ubiquitin activating enzyme	100%(21 bp)	NM121923	5	ubiquitin-like activating enzyme activity, NEDD8 activating enzyme activity
hcct-26	Mus musculus RIKEN cDNA E130307A14 gene	96%(25 bp)	XM211291	10	unknown
hcct-29	Homo. NDUFA4	98%	NM002489	7p21.3	NADH dehydrogenase activity

表2 SEREX法筛选出HCC抗原基因的功能分类

功能分类	代表基因
结构基因	HSPCA, HSP70
调节基因	EIF3S2, RAB4B, IGFBP2, RFC2, NM121923
能量代谢基因	LDH-A, NDUFA4, MCART1
未知功能基因	hypothetical protein FLJ25976, XM211291, MAGE-1, MAGE-3